PCR-ELISA检测乙型肝炎病毒DNA的临床应用  

The Clinical Application of Testing HBV-DNA with PCR-ELISA

作　　者：孟涛[1] 

机构地区：[1]黄石理工学院医学院,湖北黄石435000

出　　处：《黄石理工学院学报》2006年第1期36-37,40,共3页Journal of Huangshi Institute of Technology

摘　　要：评价PCR-ELISA(聚合酶链式反应-酶联吸附试验)测定乙型肝炎病毒DNA(HBV DNA)的临床应用价值。采用定量PCR-ELISA和ELISA法分别检测208例乙型肝炎患者血清的HBV DNA和HBV血清标志物(HBVm),34份健康体检者作为阴性对照。结果为:血清HBV DNA含量>4×106拷贝/m l分别是抗HB-cIgM组、HBeAg组、抗HBc组、抗HBe组和三抗体组(抗HBs、抗HBe、抗HBc),其HBV DNA阳性率分别为100%,97.75%,62.30%,41.67%,16.00%。PCR-ELISA法定量检测HBV DNA具有较强的特异性和灵敏度,为临床早期诊断,了解乙型肝炎病毒复制情况,判断有无传染性以及药物疗效观察具有重要的临床意义。This paper evaluates the value of the clinical application of testing HBV - DNA with PCR - ELISA. 208 HBV sufferers＇serums are tested with quantitative PCR - ELISA and HBV - ELISA. 34 healthy persons＇serums tests are done as controlled group. The result of the experiment shows that if HBV DNA in the serums is more than 4 × 106 copy/ml, the positive rate is 100%, 97.75%, 62.3%, 41.67% and 16.00% respectively in HBclgM group, anti - HBeAg group, anti - HBc group, anti - HBe group and anti - HBs, anti - HBe and anti - HBc group. PCR - ELISA quantitative test method has special features and is very sensitive, which is of important clinical significance for earlier diagnosis, learning the situation of HBV DNA viruses＇copy, judging whether the viruses are infective and observing the effectiveness of medicines.

关 键 词：PCR-EHSA 乙型肝炎病毒DNA 乙型肝炎病毒标志物 

分 类 号：R181.2[医药卫生—流行病学]