网状分枝扩增法检测EB病毒基因及临床应用研究  

Clinical significance of detection of EBV in peripheral blood of patients with NHL by ramification amplification method

作　　者：张福明[1] 李凡[2] 张淑芹[3] 

机构地区：[1]吉林大学第一医院血液科,吉林长春130021 [2]吉林大学基础医学院病原微生物教研室,吉林长春130021 [3]吉林大学再生医学研究所,吉林长春130021

出　　处：《中国实验诊断学》2006年第3期221-224,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助课题(30271251)

摘　　要：目的应用网状分枝扩增法(RAM)检测NHL外周血EB病毒基因,探讨EB病毒感染与NHL的相关性。方法利用人工合成的EBER-1启动子基因,建立网状分枝扩增检测法,以EB病毒阳性细胞株Raji和EB病毒阴性的人白血病细胞株NB4作对照,检测了NHL患者外周血中EB病毒基因。结果RAM能够检出样品中10个分子的靶基因;120例非霍奇金淋巴瘤(NHL)标本中,76例阳性(63.33%),与PCR方法检出结果相一致。结论RAM技术敏感、特异、简便易行;NHL与EB病毒感染具有较高的相关性。Objective To detect EB virus in peripheral blood of patients with NHL by ramification amplification method （RAM）. To investigate the relativity of NHL with infection caused by EB virus. Methods RAM was used to detect EB Virus with the man-made target gene sequence of promotor of EBER-1 .EB virus in some cases of patients with NHL was detected by RAM, and the result of RA M was compared with that of PCR. Raji cells and NB4 cells were used as positive control and negative control respectively. Results RAM could detect 10 molecular target gene of the exponent. 76 cases were positive in 120 cases of NHL patients and the positive rate was 63.33 %. The result is identical with that of PCR. Conclusion Ramification amplification method is sensitive,convenient and easy to perform. NHL has a high relativity with infection caused by EB virus.

关 键 词：疱疹病毒4型 非霍奇金淋巴瘤/病毒学 网状分枝扩增法 聚合酶链反应 

分 类 号：R450[医药卫生—治疗学]