腺病毒介导的可溶性Ⅰ型补体受体基因转染对急性心肌缺血的保护作用的实验研究  

Protective effects of adenovirus-mediated gene transfer of soluble complement receptor type 1 on acute myocardium ischemia: an experimental study

作　　者：黄盛东[1] 崔勇[1] 陆方林[1] 陈和忠[1] 徐静[2] 龚德军[1] 徐志云[1] 

机构地区：[1]第二军医大学长海医院胸心外科,上海200433 [2]长海医院超声科

出　　处：《第二军医大学学报》2006年第3期237-239,共3页Academic Journal of Second Military Medical University

摘　　要：目的:探讨腺病毒介导的可溶性Ⅰ型补体受体(solub le comp lem ent receptor type 1,sCR1)基因转染对急性心肌缺血的保护作用。方法:开胸阻断小鼠左冠状动脉前降支起始部30 m in后开放,实验组(14只)在开放冠状动脉前5 m in于缺血区注射携带sCR1和LacZ的混合腺病毒液(100μl,1010噬斑形成单位);对照组(13只)注射LacZ腺病毒液(100μl,1010噬斑形成单位)。术后2周时利用超声心动图检查心功能,并于检查后立即取标本行病理检查。结果:术后2周时,各项超声指标实验组都明显优于对照组(P<0.05);尤其是反映梗死范围大小的梗死范围率,差异非常显著(P<0.01)。病理检查见实验组动物心肌梗死区域小于对照组,H-E染色显示实验组注射sCR1腺病毒的范围内存活心肌细胞明显多于对照组。结论:缺血心肌再灌注前注射sCR1腺病毒可以明显减小梗死范围,提高远期心脏功能。Objective：To evaluate the protective effects of adenovirus-mediated gene transfer of soluble complement receptor type 1 （sCR1） on acute myocardium ischemia in mice. Methods： Twenty-seven SD rats were subjected to left anterior descending coronary artery （LAD） occlusion（30 min） and reperfusion. In the treatment group（n= 14）, a mixture of adenovirus carrying sCR1 and LacZ was injected into the ischemic zone（100 μl, 10^10 pfu）5 min before reperfusion; in the control group, only adenovirus carrying LacZ was injected （n= 13）. Echocardiography was performed 2 weeks later, followed immediately by pathologic examination. Results.. Echocardiographic results of the treatment group were better than those of the control group （P〈0.05） 2 weeks after the operation, especially when it comes to the size of infarction area （P〈0.01）. H-E staining showed more surviving myocardial cells in the treatment group than in the control group. Conelusion： Transfer of sCR1 gene by adenovirus can reduce the size of infarction area and preserve heart function in a long-term period.

关 键 词：心肌缺血 可溶性Ⅰ型补体受体 腺病毒 转染 

分 类 号：R542.22[医药卫生—心血管疾病]