PCR-RFLP and AP-PCR of rbcL and ITS of rDNA Show That × Taxodiomeria peizhongii （ Taxodium × Cryptomeria） Is not an Intergeneric Hybrid  

作　　者：Yan Ling Wei-Feng Lu Fan Lu Yu-Guo Wang Jia-Kuan Chen Wen-Ju Zhang 

机构地区：[1]The Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, China

出　　处：《Journal of Integrative Plant Biology》2006年第4期468-472,共5页植物学报（英文版）

基　　金：National Natural Science Foundation of China （39970061）.

摘　　要：Taxodiorneria peizhongii Z. J. Ye, J. J. Zhang et S. H. Pan was regarded as a new Intergenerlc hybrid between Taxodlum mucronatum Tenore （as the female donor） and Cryptomeria fortunei Hoolbrenk ex Otto et Dletr （as the male donor）. To confirm the authenticity of the intergeneric hybrid, we analyzed the rbcL gene and the Internal transcribed spacer （ITS） of 26S-18S ribosomal RNA gene of the three species using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and arbitrarily primed PCR （AP-PCR）, and ob- tained the following results：i） Taxodiomeria peizhongii had the same RFLP maps of the rbcL gene and the ITS as Taxodlum mucronatum, but was different from C. fortunei; II） a 311-bp PCR amplification product was obtained In C. fortunei by AP-PCR of ITS, but was not found in Taxodiomeria peizhongii. Our results have demonstrated that C. fortunei did not provide any genome for Taxodiomeria peizhongii, Implying that T. peizhongii Is not an Intergenerlc hybrid between the two species.× Taxodiorneria peizhongii Z. J. Ye, J. J. Zhang et S. H. Pan was regarded as a new Intergenerlc hybrid between Taxodlum mucronatum Tenore （as the female donor） and Cryptomeria fortunei Hoolbrenk ex Otto et Dletr （as the male donor）. To confirm the authenticity of the intergeneric hybrid, we analyzed the rbcL gene and the Internal transcribed spacer （ITS） of 26S-18S ribosomal RNA gene of the three species using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and arbitrarily primed PCR （AP-PCR）, and ob- tained the following results：i） Taxodiomeria peizhongii had the same RFLP maps of the rbcL gene and the ITS as Taxodlum mucronatum, but was different from C. fortunei; II） a 311-bp PCR amplification product was obtained In C. fortunei by AP-PCR of ITS, but was not found in Taxodiomeria peizhongii. Our results have demonstrated that C. fortunei did not provide any genome for Taxodiomeria peizhongii, Implying that T. peizhongii Is not an Intergenerlc hybrid between the two species.

关 键 词：Arbitrary Primed-Polymerase Chain Reaction （AP-PCR） intergeneric hybrid nuclear and chloroplast genome pater- nally inherited polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） × Taxodiomeria peizhongii. 

分 类 号：Q949.666[生物学—植物学]