谷胱甘肽提高月季切花失水胁迫耐性与GR活性的关系  

Effect of GSH Pretreatment on the Tolerance to Water Deficit Stress in Cut Rose'Samantha'and Related to Enhancement of GR Activity

作　　者：王子华[1] 金基石[1] 高俊平[1] 

机构地区：[1]中国农业大学观赏园艺与园林系,北京100094

出　　处：《园艺学报》2006年第1期89-94,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(39870490);科技部农业科技成果转化项目(02EFN216900751)

摘　　要：本研究确定了切花月季‘Sam antha’预处理采用的谷胱甘肽(GSH)及其生物合成抑制剂丁胱亚磺酰胺〔L-buth ione(S,R)su lfoxim ine,BSO〕适宜浓度,探讨了GSH和BSO预处理对花朵水势、花瓣中MDA含量、GSH含量以及GR活性等的影响。结果表明:与失水胁迫对照处理相比较,2 mmol/L GSH预处理明显提高了月季切花瓶插期间花朵水势,显著降低了花瓣中MDA含量,有效提高了花瓣中GSH含量和GR活性。2 mmol/L BSO预处理获得了理想的反证结果。结果说明,GSH对切花月季‘Sam antha’失水胁迫耐性的改善与花瓣中GR活性的提高相联系。In this article, the optimum pretreated concentrations of GSH （glutathione） and its biosynthetical inhibitor, BSO [ L-buthione （S, R） sulfoximine3, were determined firstly according to their effects on vase quality of the flowers after 24 h water deficit stress in cut rose （ Rosa hybrida）‘Samantha＇. Effects of 2mmol/L GSH and 2 mmol/L BSO pretreatments for 12 h on water potential of flower bud, MDA （malondialdehyde ） and GSH contents, and GR （glutathione reductase） activity in petals were investigated. The results showed that GSH pretreatment markedly increased water potential, but significantly decreased MDA content, GSH content and GR activity obviously increased during vase when compared with stress control flowers. The contrary results were obtained in 2 mmol/L BSO pretreated flowers. These results above indicate that the improvement of GSH on tolerance to water deficit stress is possibly be related to the enhancement of GR activity in petals of cut rose ＇ Samantha＇.

关 键 词：月季 切花 失水胁迫 谷胱甘肽 谷胱甘肽还原酶 

分 类 号：S685.12[农业科学—观赏园艺]