薰衣草叶片高频再生体系的建立  

Establishment of High-frequency Regeneration System from Leaf Explants of Lavandula angustifolia'Munstead'

作　　者：许耀祖[1] 韦彦余[1] 王晓军[1] 赵民安[1] 赵海清[1] 

机构地区：[1]中国科学院新疆理化技术研究所

出　　处：《园艺学报》2006年第1期182-185,共4页Acta Horticulturae Sinica

基　　金：中国科学院西部行动高新技术资助项目(KGCX2-SW-506)

摘　　要：以薰衣草叶片为外植体进行了离体再生研究。结果表明:叶片愈伤组织诱导最佳的培养基是MS+2,4-D 0.1 mg/L+6-BA 0.5 mg/L,诱导率高达100%;液体悬浮—固体培养芽分化率达92.5%,芽数/愈伤组织达6.6;正交试验筛选出芽增殖最佳培养基为MS+NAA 2.0 mg/L+6-BA 0.5 mg/L+IAA 1.0mg/L,其增殖系数高达8.7;在芽增殖培养基上可直接生根,生根率达100%。The regeneration system of Lavandula angustifolia ‘Munstead＇ in vitro was studied with leaves as explants, and the results showed the optimal medium for callus induction was MS ＋ 2, 4-D 0. 1 mg/ L ＋ 6-BA 0. 5 mg/L with the induction frequency of 100%. Liquid suspension-solid culture can achieve adventitious bud differentiation rate of 92.5% and 6. 6 bud per callus. Best bud multiplying medium which was MS medium supplemented with NAA 2. 0 mg/L and 6-BA 0. 5 mg/L and IAA 1.0 mg/L was sifted through orthogonal experiment with multiplying coefficient of 8.7. The rooting of rootless bud could be accomplished directly in the bud multiplying medium with rooting frequency of 100%.

关 键 词：薰衣草 液体悬浮-固体培养 再生植株 诱导率 增殖系数 

分 类 号：S68[农业科学—观赏园艺]