8-Br-cAMP和血小板衍生生长因子对多囊卵巢综合征患者颗粒细胞E_2生成的影响  

Effect of 8-Br-cAMP and platelet-derived growth factor on E_2 excretion of granulosa cell from polycystic ovarian syndrome patients

作　　者：贾莉婷[1] 张展[1] 王雪梅[1] 王全先[1] 袁恩武[1] 李遂柱[1] 

机构地区：[1]郑州大学第三附属医院检验科,郑州450052

出　　处：《郑州大学学报（医学版）》2006年第2期290-292,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省卫生厅创新人才基金资助项目2002214;河南省教育厅基金资助项目2003320129

摘　　要：目的探讨8-溴-环磷酸腺苷(8-Br-cAMP)和血小板衍生生长因子(PDGF)对多囊卵巢综合征(P-COS)患者离体卵巢黄素化颗粒细胞雌二醇(E2)生成的影响。方法收集体外授精-胚胎移植(IVF-ET)时PCOS患者(n=6)和正常对照(n=8)的黄素化颗粒细胞进行体外原代培养,在其培养的不同时间(0h、48h、120h)以睾酮(10-7mol/L)作为底物,分别添加8-Br-cAMP(2mmol/L)和(或)PDGF(10μg/L)于无血清培养液(TCM199)中,共培养3h后收集培养上清、细胞。用放射免疫法测定颗粒细胞分泌E2量;用考马斯亮蓝G250/BSA测颗粒细胞蛋白含量,以校正E2含量。结果与对照组比较,8-Br-cAMP和PDGF在0h、48h和120h均显著刺激PCOS组颗粒细胞分泌E2(P<0.05);8-Br-cAMP和PDGF联合作用在48h、120h显著刺激PCOS组颗粒细胞分泌E2(P<0.05)。结论8-Br-cAMP和PDGF均可加速PCOS患者卵巢颗粒细胞内雄激素向雌激素的转化;8-Br-cAMP和PDGF对颗粒细胞芳香化酶活性的调节作用是一致的。Aim ： To investigate the effects of 8-Br-cAMP and PDGF on estradiol production of the ovarian luteinized granulosa cells in vitro from PCOS patients. Methods：The luteinized granulosa cells were prepared from follicular aspirate, and pre-incubation for 0 h, 48 h, and 120 h. The test period was for 3 h in the presence of testosterone as substrate, with or without PDGF （10 μg/L） and/or 8-Br-cAMP （2 mmol/L）. At the end of the culture, the medium was collected for the measurements of estradiol by RIA, and the cells were lysed with 50 μl 0.1 mmol/L sodium hydroxide and their protein concentration were assayed by CBBG-250/BSA in order to correct E2. Results： Granulosa cells were cultured for 0 h, 48 h, and 120 h in PCOS group, the addition of 8-Br-cAMP and PDGF to the cultures for 3 h induced a significant increase in estradiol production than those of normal group（P 〈 0.05）. Granulosa cells were cultured for 48 h, and 120 h in PCOS group, the addition of 8-Br-cAMP and PDGF to the cultures for 3 h induced a significantly high increase in estradiol production than those of normal group（P 〈 0.05 ）. Conclusion ： 8-Br-cAMP and PDGF could accelerate the androgen transforming estradiol in granulose cells of ovaries from PCOS patients. The action of 8-Br-cAMP on the aromatase activity in granulose cells is consistent with that of PDGF.

关 键 词：多囊卵巢综合征 8-溴-环磷酸腺苷 血小板衍生生长因子 芳香化酶 颗粒细胞 雌二醇 

分 类 号：R711.75[医药卫生—妇产科学]