南极菲尔德斯半岛表层土壤样品中细菌多样性的系统发育分析  

PHYLOGENETIC ANALYSIS OF BACTERIAL DIVERSITY IN TOPSOIL SAMPLES FROM FILDES PENINSULA,ANTARCTICA

作　　者：李会荣[1] 孙嘉康[1] 陈丽珊[1] 俞勇[2] 陈波[2] 任大明[1] 

机构地区：[1]复旦大学遗传学研究所遗传工程国家重点实验室,上海200433 [2]中国极地研究中心国家海洋局极地科学重点实验室,上海200136

出　　处：《极地研究》2005年第4期245-254,共10页Chinese Journal of Polar Research

基　　金：复旦大学生命科学院青年创新基金项目资助

摘　　要：通过PCR结合变性梯度凝胶电泳(DGGE)技术对从南极长城站附近表层土壤样品中获得16SrDNA序列特征片段V3区序列进行分离。对其中的主要12条DGGE条带进行胶回收,获得的DNA片段经测序以及计算机比对分析发现,它们分别属于β、γ、δ-变形细菌(Proteobac-teria)、噬纤维菌-屈挠杆菌-拟杆菌(Cytophaga-Flexibacter-Bacteroides,CFB)群细菌、放线细菌(Actinobacteria)、蓝细菌属(Cyanobacteria)、酸杆菌属(Acidobacteria)和绿屈挠菌属(Chlo-roflexi)等系统分类群。南极表层土壤样品中的大部分16S rDNA序列与从其他土壤或沉积物样品中直接获得的序列相似性较高(93%—100%)。Denaturing gradient gel electrophoresis （DGGE） profiles of PCR amplified V3 regions of 16S rDNA were used to assess bacterial diversity in the topsoil samples collected in the vicinity of the Great Wall Station, Antarctica. DGGE profile showed that bac terial phylogenetic composition varied in different soils. Sequences corresponding to 12 dominating excised bands were analyzed and fell into eighl lineages of the domain Bacteria： β、γ、δ--proteobacteria; Cytophaga-Flexibacter Bacteroides（CFB）group; Actinobacteria; Acidobacteria; Cyanobacteria and green nonsulfur bacteria. The majority of the sequences were most closely related to the sequences from uncultured microorganisms in environmental （especially soils and sediments）.

关 键 词：南极 表层土壤 细菌 DGGE 16S RDNA 

分 类 号：Q931[生物学—微生物学]