家蚕sans fille基因的分子克隆及序列分析  

Cloning and Sequence Analysis of the Bombyx mori snf Gene

作　　者：赵敏[1] 刘劲[1] 朱虹[1] 查幸福[1] 夏庆友[1] 向仲怀[1] 

机构地区：[1]西南大学蚕学与生物技术学院农业部蚕桑学重点开放实验室,重庆400716

出　　处：《蚕业科学》2006年第1期6-11,共6页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金重点项目(编号30330460);国家重点基础研究发展计划"973"项目(编号2005CB121000);国家科技攻关项目(编号2005BA711A07)

摘　　要：生物性别决定是由一组基因通过级联网络来调控。果蝇sans fille(snf)基因在其性别决定过程中具有重要作用。结合生物信息学与分子生物学实验,成功克隆了家蚕的snf基因(Bm snf基因)。克隆的Bm snfcDNA全长915 bp,ORF长630 bp,编码209个氨基酸。该基因推定蛋白质的预测分子量为24 kD,等电点为9.75。将Bm snf的cDNA与家蚕基因序列进行比对,结果表明该基因具有5个外显子,外显子/内含子边界处均符合GT-AG规则。比较家蚕snf和果蝇snf基因,显示两者编码氨基酸序列相似性为69.9%。通过SMART软件分析,家蚕snf和果蝇snf的RRM很相似。以上结果表明,家蚕snf很可能发挥了类似果蝇snf的作用。The cascade of sex determination in organism was regulated by a set of genes. The gene sans fille （snf） of Drosophila plays an important role in its sex determination, We cloned Bmsnf successfully through bioinformatJcs and biological experJment. The length of the Bmsnf cDNA was 915 bp, the ORF （Open Read Frame） included 630 bp in length and encoded 209 amino acids, with the predicted molecular weight of 24, 0 kD and isoelectric point of 9.75. An alignment of the cDNA sequence with the silkworm genome sequences revealed that there were 5 exons in Bmsnf and all of them conformed to the GT-AG rule. Compared Bmsnf with Drosophila snf, they share 69.9% similarity at amino acid level. The results indicate that Bmsnf might have the similar function with drosophila snf.

关 键 词：家蚕 性别决定 snf基因 剪接因子 

分 类 号：S881[农业科学—特种经济动物饲养]