家蚕5龄幼虫不同时期中部丝腺细胞蛋白质双向电泳分析  

Investigation on Protein Variety in Middle Silkgland Cells of Silkworm,Bombyx mori,During Fifth Instar Stage

作　　者：沈飞英[1] 钟伯雄[1] 楼程富[1] 洪波[1] 蒋振东[1] 苏松坤[1] 梁建设[2] 徐海圣[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029 [2]浙江大学生物化学有限公司,杭州310004

出　　处：《蚕业科学》2006年第1期25-35,共11页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划"973"项目(编号2005CB121003);浙江省科技厅项目(编号2004C32048)

摘　　要：对5龄家蚕不同发育时期的中部丝腺细胞蛋白质变化进行分析研究,有利于发现与丝胶蛋白分泌有关的功能蛋白质。采用蛋白质双向电泳和图像分析技术,发现同一区段的家蚕中部丝腺细胞在5龄第2天、第4天和熟蚕期的蛋白质组成具有显著差异:中部丝腺前部区段5龄第2天表达的特异性蛋白质斑点有8个,第4天有16个,熟蚕期有24个;中部区段5龄第2天表达的特异性蛋白质斑点有9个,第4天有33个,熟蚕期有30个;后部区段5龄第2天表达的特异性蛋白质斑点有10个,第4天有7个,熟蚕期有25个。Investigation on the protein varieties in the middle silkgland cells of silkworm, Bombyx mori, during 5^th instar stage could find out the functional proteins concerning with the secretion of the sericin protein. Using two dimensional polyacrylamide gel electrophoresis （2D-PAGE） and the image analysis technology, the results indicated that a significant difference in the protein composition of the same part of middle silkgland cells among 2^nd day, 4^th day and the mature stage during 5^th instar. Eight special protein spots were expressed in anterior part of middle silkgland cells in 2^nd day , 16 in 4^th day and 24 in the mature stage. In middle part of middle silkgland cells, 9, 33, and 34 especial protein spots were detected in 2^nd day, in 4^th day and in the ma- ture stage, respectively. In posterior part of middle silkgland cells, 10, 7, and 25 especial protein spots were found out in 2^nd day, in 4^th day and in the mature stage, respectively.

关 键 词：家蚕 中部丝腺 丝胶蛋白 蛋白质双向电泳 蛋白质图谱分析 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q51[农业科学—畜牧兽医]