家蚕不同品种后部丝腺蛋白质双向电泳图谱比较  

Analysis of 2D-PAGE Patterns of Proteins in Posterior Silkgland From Different Silkworm Varieties on 4^(th) Day in 5^(th) Instar

作　　者：鲁华云[1] 叶键[1] 李建营[1] 钟伯雄[1] 

机构地区：[1]浙江大学动物科学学院蚕蜂分子生物学实验室

出　　处：《蚕业科学》2006年第1期114-117,共4页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划973项目(编号2005CB121003);浙江省科技厅项目(编号2004C32048)

摘　　要：采用蛋白质双向电泳和图像分析技术,研究发现不同品种的家蚕5龄第4天后部丝腺细胞的蛋白质组成存在明显差异,并且蚕茧高产品种与蚕茧中、低产量品种之间所涉及的差异蛋白质斑点各不相同,暗示丝素基因的转录、翻译可能涉及了一个非常复杂的多点控制的调控系统,即不同品种的产茧量可能涉及不同的调控位点或调控因子。Using two-dimensional electrophoresis of protein and image analysis system, we discovered that protein constituents in the posterior silkgland on 4^th day in 5^th instar are apparently different for different silkworm varieties. Comparing with those in silkworm variety with the middle, low yield cocoon, we found that there existed many different protein spots in silkworm variety with the high yeild cocoon. This suggested that the transcription and translation of fibroin gene might be involved in a complex regulation system with a great deal of regulation points, and the yeild cocoon in different varieties may be related with different regulation points or factors.

关 键 词：家蚕 后部丝腺 蛋白质 双向电泳 图谱分析 

分 类 号：S881[农业科学—特种经济动物饲养]