家蚕核糖体的提取及其Sarcin/Ricin结构域的初步鉴定  

Ribosome Isolated From Silkworm,Bombyx mori,and Preliminary Identification of its Sarcin/Ricin Domain

作　　者：魏国清[1] 代君君[1] 陈复生[2] 刘朝良[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]安徽省农业科学院蚕桑研究所,合肥230061

出　　处：《蚕业科学》2006年第1期118-121,共4页ACTA SERICOLOGICA SINICA

基　　金：安徽省自然科学基金项目(编号03041406);安徽省高等学校青年教师资助计划项目(编号2003jq116);安徽省2005年度科研重点项目(编号05021003)

摘　　要：用超速离心结合密度梯度离心法从蚕蛹中提取家蚕80S核糖体,经尿素变性聚丙烯酰胺凝胶电泳,结果表明家蚕核糖体的RNA同大鼠核糖体RNA在序列长度上有明显差异,家蚕5.8S rRNA高于大鼠5.8S rRNA,而家蚕5S rRNA的序列长度则略低于大鼠。用核糖体失活蛋白R ic in分析鉴定家蚕核糖体的Sarc in/R ic in结构域,结果是家蚕Sarc in/R ic in结构域比大鼠Sarc in/R ic in结构域离28S rRNA的3′末端更近。The 80S ribosome of silkworm,Bombyx mori, is isolated from the pupae by ultracentrifugation and gradient centrifugation.The ribosomal RNA is determined by the urea-denatured PAGE,and the results suggests that the rRNA of silkworm is different from those of rat.5.8S rRNA of silkworm is bigger and 5S rRNA is smaller than that of rat,respectively.Furthermore,using Ricin as probe,the Sarcin/Ricin domain is identified.The results show that the silkworm Sarcin/Ricin domain is nearer to the 3′-end of 28S rRNA than that of rat.

关 键 词：家蚕 核糖体 Sarcin/Ricin结构域 

分 类 号：S881[农业科学—特种经济动物饲养]