槲皮素对血吸虫肝纤维化小鼠肝组织c-fos、c-jun mRNA和转化生长因子β1表达的抑制作用  

Inhibition of quercetin on expressions of c-fos, c-jun mRNA, and TGF-β1 in liver tissue of hepatic fibrosis mice infected by schistosome

作　　者：徐标[1] 韩春荣[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院感染科,湖北武汉430022

出　　处：《中草药》2006年第3期393-398,共6页Chinese Traditional and Herbal Drugs

摘　　要：目的研究槲皮素对小鼠血吸虫病肝纤维化的治疗作用及其机制,并与吡喹酮的作用进行对比。方法80只小鼠随机分为4组。其中3组小鼠于感染日本血吸虫8周后,分别以槲皮素30mg/(kg·d)治疗8周、以吡喹酮500mg/(kg·d)治疗2d及不作任何治疗(模型组),第4组小鼠作为正常组。应用HE染色法、RT-PCR及免疫组化染色,观察和分析槲皮素及吡喹酮治疗前后各组小鼠肝组织病理改变及c-fos和c-junmRNA、转化生长因子β1(TGF-β1)和、型胶原表达的变化。结果槲皮素治疗后小鼠肝纤维化程度减轻,与模型组相比,肝组织中c-fos、c-junmRNA以及TGF-β1和、型胶原表达均明显降低(P<0.01);与吡喹酮组相比,c-junmRNA、和型胶原表达明显降低(P<0.05),而c-fosmRNA和TGF-β1表达无显著改变。结论槲皮素对小鼠血吸虫病肝纤维化具有较好的治疗作用,其远期抗肝纤维化效果优于吡喹酮。Objective To study the therapeutical effects and mechanisms of quercetin on hepatic fibrosis in schistosome-infected mice and compare the effects with praziquantel. Methods Eighty mice were divided into four groups： among them three groups were infected by Schistostrma japonica. After 8 weeks, one group was treated with quercetin 30 mg/（kg · d） for 8 weeks, one group was treated with praziquantel 500 mg/（kg · d） for 2 d and the other was taken as model group without any treatment. The fourth group was taken as normal group. HE staining, RT-PCR, and immunohistochemical technique were used to observe the expression of hepatic c-los and c-jun mRNA, and the changes of hepatic TGF-β1, type Ⅰ , and type Ⅱ collagen in mice infected by S. japonica before and after treatments. Results Quercetin obviously relieved the degree of hepatic fibrosis, significantly reduced the expression of c-los, c-jun mRNA, TGF- β1, type Ⅰ , and type Ⅲ collagen compared to the model group （P〈0.01）, and the expression of c-jun mRNA, type Ⅰ , and type Ⅲ collagen compared to the group treated with praziquantel （P〈0.05）, whereas no difference in the level of c-los mRNA and TGF-β1 between the two groups. Conclusion Quercetin has better therapeutical effects on hepatic fibrosis in schistosome-infected mice and surpasses praziquantel in long term.

关 键 词：槲皮素 日本血吸虫 肝纤维化 c—fos C-JUN 转化生长因子-β1 

分 类 号：R287.86[医药卫生—中药学]