祛湿化瘀方对CCl_4复合高脂低蛋白饮食诱导的大鼠脂肪肝的防治作用  

Prevention and Treatment of "Qushi Huayu Decoction" on Fatty Liver of Rats Induced by Carbon Tetrachloride Along with High-Fat and Low-Protein Diet

作　　者：张慧[1] 冯琴[1] 胡义扬[1] 刘成海[1] 刘平[1] 

机构地区：[1]上海中医药大学附属曙光医院上海中医药大学肝病研究所,上海201203

出　　处：《上海中医药杂志》2006年第3期52-55,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市经委基金资助项目(2003-B-011);上海市教委重点学科建设项目(Y0302);上海市中医肝病临床医学中心研究基金资助

摘　　要：目的探讨中药祛湿化瘀方防治非酒精性脂肪肝的疗效及作用机制。方法将Wistar雄性大鼠采用四氯化碳(CCl4)皮下注射及高脂低蛋白饮食诱导,复制大鼠脂肪肝模型在造模2周后,随机分模型组、祛湿化瘀方组及甘乐对照组。用药后,观察肝组织病理变化,肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量和谷氨酰转肽酶(GGT)、超氧化物歧化酶(SOD)活性,血清肝功能和血脂指标及肿瘤坏死因子α(TNF-α)含量和肝组织TNF-α蛋白表达。结果模型组肝组织TG、FFA含量分别达正常组的5.3、1.9倍,肝组织出现严重的大泡样脂肪变性,肝脏炎症表现明显;祛湿化瘀方组的上述改变显著减轻,其肝组织TG、FFA含量显著低于模型组。同时可显著降低在模型组中异常升高的肝组织GGT活性及ALT、AST活性,显著提高模型组中低下的肝组织SOD活性,并且可显著降低血清TNF-α含量和肝组织中TNF-α蛋白的表达;对照组主要表现为有显著的抗肝损伤作用,其肝组织TG、FFA含量变化与模型组比较无显著性差异。结论祛湿化瘀方对CCl4高脂低蛋白饮食诱导的脂肪肝及其炎症有理想的防治作用。Objective To discuss the experimental efficacy and mechanisms of ＂Qushi Huayu Decoction＂ on prevention and treatment of non-alcoholic fatty liver （NAFL）. Methods Wistar male rats were used to establish NAFL model by carbon tetrachloride （CC14） subcutaneous injection for 4 weeks（twice per week） along with high-fat and low-protein diet for 2 weeks. After two weeks, the rats were respectively divided into model group, Qushi Huayu Decoction group and Ganle treated comparison group at random. After the period of treatment, the followings were detected （1） liver pathology; （2）the content of liver triglyceride（TG）, free fatty acid （FFA） and the activity of liver 3,-glutamyltransferase （GGT）; （3）serum tumor necrosis factor-alpha content, amino- transferase values; （4） TNF-α protein expression. Results In the experiment, liver content of TG and FFA of the model group were significantly increased compared with the normal control group （the content of TG and FFA is 5.3 times and 1.9 times as that of the normal control group respectively）; and there were serious hepatic steatosis and inflammation in model group. But all of them were decreased significantly in Qushi Huayu Decoction group, ＂Qushi Huayu Deooction＂ can also significantly decrease liver activity of GGT and serum ALT, AST activity and increase liver activity of SOD. Furthermore, ＂Qushi Huayu Decoction＂ can significantly decrease serum TNF-α content and TNF-α protein expression. Ganle had good anti-liver damnification effect, but it had no effect on liver content of TG and FFA. Conclusion Qushi Huayu Decoction has good experimental efficacy on NAFL induced by carbon tetraehloride along with high-fat and low-protein diet.

关 键 词：祛湿化瘀方 非酒精性脂肪肝 中医药疗法 动物实验 

分 类 号：R285.5[医药卫生—中药学]