利用基因诱捕识别哺乳动物发育调控基因  

Using Gene Trap Screen to Indentify Developmentally Regulated Genes in Mammals

作　　者：曾玮青 孙方臻[1] 

机构地区：[1]中国科学院发育生物学研究所

出　　处：《生物化学与生物物理进展》1996年第3期194-200,共7页Progress In Biochemistry and Biophysics

摘　　要：ＥＳ细胞是一种来源于胚胎的多潜能细胞，它可在体外培养并进行基因操作，而且通过囊胚注射制作嵌合体的途径，能将外源基因掺入小鼠的基因库中，因此利用ＥＳ细胞可筛选出发生基因突变的小概率事件并获得其遗传突变体．利用基因诱捕载体与ＥＳ细胞，研究与哺乳动物发育调控有关的未知基因，这一新技术将成为阐明胚胎发育过程中基因表达的时空格式的有效手段．ES cells are early embryo-derived multipotential cells that can be genetically manipulated in tissue culture. Thus genetic modification can be introduced into the gene pool by injecting transfected ES cells into blastocysts.The unique advantage that ES cells offer is thatthey can be screened in culture for rare geneticevents prior to germline transmission and the desired genetic mutant can be produced.Recently, genes which are involved in mousedevelopment can be identified using gene trapvectors in combination with ES cells. This newapproach is very efficient for studying geneexpression pattern during embryogenesis.

关 键 词：哺乳动物纲 基因诱捕 发育调控基因 

分 类 号：Q959.803[生物学—动物学]