Vero细胞森林脑炎疫苗毒种的选育及其生物学特性检测  

Screening and Biological Characteristics of Virus Seed for Preparation of Vero cell-derived Tick-borne Encephalitis Vaccine

作　　者：宋宗明[1] 刘双军[1] 王丽娜[1] 赵洪丽[1] 王伟[1] 刘景云[1] 洪成龙[1] 惠连[1] 李彤[1] 韩亮[1] 

机构地区：[1]长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》2006年第1期52-53,共2页Chinese Journal of Biologicals

基　　金：国家"十五"重大科技"863"专项(2004AA2Z3630)

摘　　要：目的选育Vero细胞森林脑炎疫苗适应毒种。方法将森林脑炎疫苗鼠脑病毒“森张”株在Vero细胞上连续传代适应,并对不同代次收获的病毒液的病毒滴度、免疫原性、稳定性等进行检测。结果“森张”株病毒在Vero细胞上传代适应后,病毒滴度达8·5LgLD50/ml;免疫血清中和抗体效价高于1∶20,其它检测项目均符合疫苗生产用毒种的要求。结论Vero细胞适应的“森张”株毒种的初步特性显示可作为制备Vero细胞森林脑炎疫苗用毒种。Objective To screen an appropriate virus seed for the preparation of Vere cell-derived tick-borne encephalitis vaccine. Methods The current virus strain Sen-Zhang for preparation of tick-borne encephalitis vaccine was subeultured in Vere cells, and the titers,immunogenieities and stabilities of vires of various passages were studied. Results The virus titer of Sen-Zhang strain adapted in Vere cells reached 8.5 LgLD50/ml. The serum neutralizing antibody titer of mice immunized with the strain reached more than 1： 20. All the quality indexes of the adapted strain met the requirements for vires seeds for vaccine produetion. Conclusion The Vere cell-adapted Sen-Zhang stain might be used as a vires seed for preparation of Vere cell-derived tick-borne encephalitis vaccine.

关 键 词：毒种选育 VERO细胞 免疫原性 生物学特性 

分 类 号：R512.990.3[医药卫生—内科学] R512.340.1[医药卫生—临床医学]