黄芪有效成分对氧自由基清除作用的ESR研究  

Scavenging Effects of Active Principles from Astragalus mongholicus Bunge on Oxygen Free Radicals Studied by ESR

作　　者：汪德清[1] 沈文梅[1] 田亚平[1] 孙仲怡[1] 丛建波[1] 吴可[1] 

机构地区：[1]解放军总医院生化科,军事医学科学院放射医学研究所

出　　处：《生物化学与生物物理进展》1996年第3期260-262,共3页Progress In Biochemistry and Biophysics

摘　　要：用电子自旋共振技术研究了黄芪总黄酮（ＴＦＡ）、黄芪总皂甙（ＴＳＡ）和黄芪总多糖（ＴＰＡ）对次黄嘌呤／黄嘌呤氧化酶体系产生的超氧阴离子自由基和Ｈ２Ｏ２－Ｆｅ２＋体系产生的羟自由基的清除作用．结果表明，这３种成分均有清除氧自由基的作用；对超氧阴离子自由基的清除效能大于对羟自由基的清除作用；其作用强度依次为ＴＦＡ＞ＴＳＡ＞ＴＰＡ．结果提示清除氧自由基可能是黄芪抗衰老的主要机理之一，ＴＦＡ和ＴＳＡ是黄芪抗氧化作用的主要药理活性成分．Scavenging effects of total flavonoids of Astragalus (TFA), total saponins of Astragalus (TSA) and total polysaccharide of Astragalus (TPA) on superoxide anion radical and hydroxyl radical have been studied by ESR method. The results showed that all the three principles have stronger scavenging effect on superoxide anion radical than on hydroxyl radical. The intensity of scavenging effect of the three principles was TFA>TSA>TPA. It is suggested that the TFA and TSA are the main component in Astragalus mongholicus Bunge for protection against free radical damages.

关 键 词：中药 黄芪 电子自旋共振 氧自由基 有效成分 

分 类 号：R282.710.5[医药卫生—中药学]