钙和生长素对棉花幼苗侧根发生的协同调控效应  

Effects of Calcium and Auxin on Lateral Roots Initiation of Cotton Seedling

作　　者：谢志霞[1] 张一[1] 田晓莉[1] 李召虎[1] 何钟佩[1] 翟志席[1] 王保民[1] 段留生[1] 

机构地区：[1]中国农业大学作物化学控制研究中心,北京100094

出　　处：《棉花学报》2006年第2期99-103,共5页Cotton Science

基　　金：国家自然科学基金(30200169);高校博士点基金(20010019008)资助项目

摘　　要：通过对陆地棉中棉所29幼苗主根与下胚轴交接处进行硝酸钙、吲哚丁酸（IBA）、Ca^2＋通道拮抗剂LaCl3和Ca^2＋螯合剂乙二醇-双-（2-氨基乙基）四乙酸（EGTA）、钙调素（CaM）拮抗剂三氟啦嗪（TFP）、以及生长素极性运输抑制剂三碘苯甲酸（TIBA）处理，研究了Ca^2＋和IBA对棉花根系的影响。50mg·L^-1Ca^2＋或5mg·L^-1 IBA都增加棉花侧根数，但抑制主根伸长；Ca^2＋与IBA协同作用促进侧根同时缓解单因子对主根伸长的抑制。5mg·L^-1 IBA处理增加了主根基部IAA含量，降低了主根根尖和中部IAA及赤霉素（GAs）含量，主根各区段细胞分裂素（CTKs）含量均增加且IAA／CTKs比例显著降低。3～10mg·L-^1 TIBA处理抑制侧根发生和促进主根伸长，降低主根各区段CTKs含量，降低主根基部和中部吲哚乙酸（IAA）含量和IAA／CTKs比例，增加根尖IAA含量和IAA／CTKs比例。3～10mg·L^-1的LaCl3、EGTA和TFP均抑制侧根发生和主根伸长，同时降低主根中游离CaM含量。Effects of Ca^2＋ and auxins on the root growth of cotton were studied using Gossypium Hirsutiurn L. cv. CCRI 29. The cotton seedlings were cultured in two layers of filter paper adhered on glass board with lower 2 cm dipped in water and grown in growth chamber at 25±1℃. The agar solution dissolving designed concentration of Ca （NO3）2, indolebutyric acid （IBA） , Ca （NO3）2-＋IBA, Ca^2＋ antanogists or calmodulin （CAM） antanogists was respectively pasted on the conjunction area of seedling hypocotyl and radicle. Treatments of 50 mg·L^-1 Ca （NO3）2 and 5 mg·L^-1 IBA promoted the lateral roots initiation （LRI） by 14. 8% and 29.6%, whereas retarded the primary root elongation （PRE） by 13.4% and 8.3% respectively. 50 mg·L^-1 Ca^2＋ ＋5 mg·L^-1 IBA showed synergic effects as the LRI was 33% higher than CK while the PRE was close to CK and higher significantly than Ca （NO3）2 or IBA treatment solely. All the PRE,LRI and free CAM content were inhibited by the treatments of 3-10 mg·L^-1 LaCl3 （Ca^2＋ inhibitor, 3-10 mg·L^-1 EGTA（calcium chalator） and 3-10 mg·L^-1 TFP （CAM antanogist）. 5 mg·L^-1 IBA enhanced IAA in basal radicle segment,repressed IAA and GAs in appical and middle segment of radicle,heightened CTKs content and decreased IAA/CTKs in all segments of radical. 3-10 mg·L^-1 TIBA reduced CTKs in whole radicle,IAA 和 IAA/CTKs in basal and middle segment,while increased IAA content and IAA/CTKs in radicle apical,which might be contributed to its restrainment on LRI and enhancement on PRE.

关 键 词：棉花 生长素 CA^2+ 侧根 调控 

分 类 号：S562[农业科学—作物学]