SARS-CoV-X4蛋白在SARS患者肺组织中的表达及其意义  

Expression and significance of severe acute respiratory syndrome associated coronavirus (SARS-CoV)-X4 protein in lungs of SARS patients

作　　者：谭亚夏[1] 陈涛[1] 钟淑卿[1] 黎毅敏[1] 陈英玉[2] 邱晓彦[2] 莫小宁[2] 宋泉声[2] 甘启倪[2] 顾莹莹[1] 莫红缨[1] 罗炜[1] 何新明[1] 刘慕嫦[1] 钟南山[1] 

机构地区：[1]广州医学院第一附属医院广州呼吸疾病研究所,510120 [2]北京大学人类疾病基因研究中心

出　　处：《中华内科杂志》2006年第3期196-198,共3页Chinese Journal of Internal Medicine

基　　金：国家自然科学基金资助项目(30370622);广东省教育厅SARS研究基金(2003-12);广东省自然科学基金重点项目(020741);国家"973"研究基金(2003CB514109)

摘　　要：目的探讨SARS-CoV-X4蛋白在SARS患者肺病变组织中的表达及其意义。方法取4例SARS患者的肺组织,HE染色观察肺组织病理学变化,免疫组化检测肺病变组织中SARS-CoV-X4蛋白表达。结果(1)HE染色可见肺组织充血、水肿,肺泡腔内大量红细胞及纤维渗出,透明膜形成。肺泡上皮变性坏死脱落,部分肺组织纤维细胞明显增生。(2)4例SARS患者肺组织损伤病变中可见黄棕色SARS-CoV-X4蛋白高表达,弥漫分布在肺泡上皮、红细胞、白细胞及渗出液中。结论SARS-CoV-X4蛋白可能参与了SARS的发生与发展。SARS-CoV的传播途径除呼吸道外,尚可能通过血液、呼吸道分泌物等传播。Objective To investigate the significance of severe acute respiratory syndrome associated coronavirus （SARS-CoV）-X4 protein expression in lungs of patients with SARS. Methods Pathological features of the lungs from 4 SARS patients were examined and the expression of SARS-CoV-X4 protein in the lungs was evaluated with immunohistochemical staining using specific antibodies against protein X4. Results Microscopically, all lungs from 4 cases showed edema, erythrocyte and fibrin exudates in the alveoli, hyperplasia of alveolar epithelium, necrosis, hyaline membrane formation and fibroblast foci. Immunohistochemical stains showed a strong positivity of X4 protein in denudation cells, vascular endothelial cells and also erythrocytes and neutrophils in the alveoli of the lung tissues from the 4 cases. Condusions Expression of SARS-CoV-X4 protein in the lungs may be involved in the pathogenesis and progression of SARS.

关 键 词：严重急性呼吸综合征 肺 冠状病毒 SARS-CoV-X4蛋白 

分 类 号：R511.9[医药卫生—内科学]