鼠伤寒杆菌主要外膜蛋白作为保护性抗原的研究  

Study on Major Outer Membrane Proteins of Salmonella Typhimurium as a Protective Antigen

作　　者：赵跃武[1] 赵风兰[1] 徐友梅[1] 胡军[1] 臧郑菊[1] 

机构地区：[1]河南医科大学微生物学与免疫学教研室

出　　处：《微生物学免疫学进展》1996年第1期11-14,共4页Progress In Microbiology and Immunology

摘　　要：采用超声破碎，ＴｒｉｔｏｎＸ─１００处理和Ｓｅｐｈａｃｒａｌ超细Ｓ─３００凝胶过滤技术提取了鼠伤寒杆菌的主要外膜蛋白（ＭＯＭＰｓ）。ＭＯＭＰｓ的脂多糖（ＬＰＳ）含量约为０．２％。经ＳＤＳ─ＰＡＧＥ图谱显示蛋白在３６─４１ＫＤ之间。ＭＯＭＰｓ能使小鼠产生典型的足垫肿胀（ＤＴＨ）及高水平的ＩＬ─２；可保护５００ＬＤ５０鼠伤寒杆菌及伤寒杆菌的攻击，其免疫保护率分别为９０％和３３．３％，用５０ｕｇＭＯＭＰｓ免疫的小鼠的Ｔ淋巴细胞经尾静脉注射给非免疫小鼠，可使后者得到被动免疫保护，其保护率为４２．９％。基于上述实验结果，本文认为鼠伤寒杆菌的ＭＯＭＰｓ是一良好的保护性抗原。Major outer membrane proteins (MOMPs) from Salmonella typhimurium were extracted by using super sonication and Sephacral fine s-300, found to contain 0.2% lipopolyaccharide (LPS) approximately. MOMPs were shown 5 bands by SDS-PAGE. In MOMPs immunized BALB/c mice significant level of delayed-type hypersensitivity (DTH) response and interleukin-2(IL-2) production were detected. Their ablities of inducing protective immunity against challenge with the bacteria were studied in BALB/c mice. Active immunization with MOMPs induced 90% protection to a intraperitoneally (i, p) challenge with 500 LD50 of Salmonella typhimurium in BALB/c mice. In addition,33.3% cross-protection against challenge with 500 LD50 of Salmonella typhi was also achieved. Furthermore,We found that T cells from the MOMPs-immmunized mice could transfer the protection (71.4%) against Salmonella typhimurium to nonimmunized mice. These resultes suggest that STM-MOMPs can be a good protective antigen.

关 键 词：鼠伤寒杆菌 外膜蛋白 保护性抗原 

分 类 号：R378.23[医药卫生—病原生物学] R392.11[医药卫生—基础医学]