昆明白族儿童幽门螺杆菌感染与HLA-DQA1等位基因的相关性  

Association between HLADQA1 alleles and H pylori infection in Bai-ethnic children from Kunming

作　　者：王美芬[1] 黄永坤[1] 郝萍[2] 戚勤[1] 文革生[1] 李海林[1] 周丽芳[1] 

机构地区：[1]昆明医学院第一附属医院儿科,云南省昆明市650032 [2]昆明医学院第一附属医院临床医学实验中心,云南省昆明市650032

出　　处：《世界华人消化杂志》2006年第4期442-445,共4页World Chinese Journal of Digestology

基　　金：云南省教育厅重点项目基金;No.04Z065C

摘　　要：目的:探讨昆明白族儿童HLA-DQA1等位基因位点上是否存在幽门螺杆菌(H pylori)感染的易感基因或抵抗基因,研究H pylori感染的免疫遗传机制．方法:应用聚合酶链反应-序列特异性引物 (PCR-SSP)方法对应用胶体金标疫渗滤法 (DIGFA)和免疫印迹法联合诊断的32例H pylori感染昆明白族儿童(实验组)和34例无 H pylori感染的昆明白族儿童(对照组)进行 HLA-DOA1基因分型检测．结果:实验组HLA-DQA1*010401或*0402基因频率明显低于对照组,两组比较差异存在显著性(0 vs 15％,X2=0,P=0．001,Pc=0．014, OR=1．172,95％CI:1．062-1．294)．结论:HLA-DQA1*010401或*0402基因可能对H pylori感染具有免疲抵抗作用．AIM： To find the susceptible or resistant genes of H pylori infection in Bai-ethnic children of Kunruing, and to explore the immunogenetic mechanism of H pylori infection. METHODS： Polymerase chain reaction-sequence specific primers （PCR-SSP） technique was used to detect the allelic distribution of HLA-DQA1 in Bai-ethnic children with or without Hpylori infection （n = 32, 34, respectively） from Kunming. H pylori infection was identified by combination of dot-immunogold filtration assay and immunobloting technique. RESULTS： The allelic frequency of HLA-DQA1^＊010401 or^＊0402 was significantly lower in children with H pylori infection than that in children without Hpylori infection （0 vs 15%,χ^2 = 0, P = 0.001, Pc = 0.014, OR = 1.172, 95%CI： 1.062-1.294）. CONCLUSION： HLA-DQA1^＊010401 or ^＊0402 may be resistant gene of Hpylori infection in Bai-ethnic children from Kunming.

关 键 词：白族儿童 幽门螺杆菌 HLA-DQA1 

分 类 号：R725.7[医药卫生—儿科]