细胞外基质金属蛋白酶诱导因子在巨噬和泡沫细胞中的表达  

Expression Profile of EMMPRIN in Macrophages and Foam Cells

作　　者：张俊峰[1] 葛恒[1] 郭炳诗 邵琴[1] 王长谦[1] 

机构地区：[1]上海交通大学医学院仁济医院心内科,上海200001 [2]中国科学院上海生命科学院上海交通大学医学院健康科学研究所

出　　处：《上海交通大学学报（医学版）》2006年第3期225-228,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30070869)资助项目

摘　　要：目的观察细胞外基质金属蛋白酶诱导因子(EMMPRIN)在单核向巨噬和泡沫细胞分化过程中的表达变化。方法体外诱导THP-1单核细胞分化为巨噬和泡沫细胞,应用Real-time RT-PCR和Western blot测定EMMPRIN基因和蛋白表达。结果单核细胞分化为巨噬细胞后EMMPRIN mRNA和蛋白表达均显著增加,当进一步分化为泡沫细胞时,EMMPRIN的表达与巨噬细胞比较无显著性差异。结论单核细胞向巨噬细胞的分化诱导EMMPRIN表达,而进一步向泡沫细胞分化对其表达无影响。Objective To investigate the expression profile of extracellular matrix metalloproteinase inducer （EMMPRIN） in macrophages and foam cells. Methods THP-1 monocytes were differentiated into macrophages in vitro, and then induced into foam cells by culturing with oxidized low density lipoproteins （oxLDLs）. Real-time RT- PCR and Western blot were performed to detect the expression of EMMPRIN genes and protein. Results The expressions of both EMMPRIN mRNA and protein were significantly increased during phorbol 12-myristate 13-acetate （ PMA）-induced monocyte differentiation into macrophages. However, the same results were not observed when macrophages were further induced into foam cells by oxLDLs. Conclusion Our findings revealed an upregulation of EMMPRIN during monocyte differentiation into macroPhages and foam cells.

关 键 词：细胞外基质金属蛋白酶诱导因子 基质金属蛋白酶 巨噬细胞 泡沫细胞 

分 类 号：R543.5[医药卫生—心血管疾病]