平原条件下训练与低氧舱睡眠对生长期大鼠骨形成及骨吸收相关因子的影响  

Effect of exercises on the plain and sleep in the hypoxia cubage on bone formation and absorption related factors in growing rats

作　　者：张红品[1] 王维群[2] 张林[2] 丁宁炜[3] 

机构地区：[1]临沂师范学院体育系,山东省临沂市276005 [2]苏州大学体育系,江苏省苏州市215021 [3]江苏省体育科学研究所,江苏省南京市210014

出　　处：《中国临床康复》2006年第13期73-75,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察白天在平原条件下照常训练,晚间在低氧舱睡眠的“高住低练”条件对生长期大鼠骨代谢的影响。方法:实验于2003-09/12在江苏省体育科学研究所动物实验室进行,取8周龄SD纯系雄性大鼠70只,经跑台练习后,选取跑动正常的大鼠55只随机分为6组:①低氧对照组(n=10):20:00～次日8:00在低氧(体积分数为0.147的氧)帐篷中休息,不运动。②低氧中负荷组(n=9):白天进行平坡跑(26.8m/min,60%～70%最大吸氧量,30min/次,5d/周,共9周),睡眠同低氧对照组。③低氧大负荷组(n=12):白天进行上坡跑(15°)强度为80%～90%最大吸氧量,速度、训练时间及睡眠同低氧中负荷组。④常氧对照组(n=7)、常氧中负荷组(n=9)和常氧大负荷组(n=8),运动方式同相对应的低氧组,晚上在常氧(体积分数为0.21的氧)中休息。9周后测定大鼠骨形成生化标志物血清碱性磷酸酶活性和骨吸收生化标志物血清抗酒石酸盐酸性磷酸酶活性、尿羟脯氨酸和肌酐等指标。结果:55只大鼠进入结果分析。①血清碱性磷酸酶:常氧大、中负荷组均高于常氧对照组;常氧和低氧组内,大负荷组均低于中负荷组;低氧对照组高于常氧对照组;低氧大、中负荷组均低于常氧对照组,大负荷组低于中负荷组。②血清抗酒石酸盐酸性磷酸酶活性:常氧大、中负荷组低于常氧对照组和低氧大、中负荷组;低氧组中大负荷组高于中负荷和对照组。③尿羟脯氨酸/肌酐:常氧大、中负荷组和低氧对照组均低于常氧对照组,低氧大、中负荷组均高于常氧对照组。结论:常氧运动和低氧可促进成骨细胞的骨形成、抑制破骨细胞的骨吸收,对生长期大鼠骨量增长有益。而“高住低练”的训练效果却相反。提示“高住低练”的训练模式对少儿骨发育不利。AIM： To observe the effect of normal exercise on the plain by day and sleep in the hypoxia cubage （Hilo） at night on bone metabolism of growing rats. METHODS： The experiment was conducted at the animal laboratory of Research Institute of Sports Science of Jiangsu Province from September to December 2003. Totally 70 purified male SD rats, aged 8 weeks were enrolled. Altogether 55 rats which were normal in running exercise on the table were chosen and randomly divided into 6 groups ： ① Hypoxia control group（Group A） （n=10）, in which , the rats rested in the hypoxia （0.147 volume fraction oxygen ）in the tabernacle without movement from 20：00 on the same day to 8：00 on the next day. ②The group of training of middling exercise tolerance in hypoxia （Group B）（n=9）： Running in smooth grade by day （26.8 m/min, 60%-70% VO2 max, 30 min/time, 5 days/week ,9 weeks in total）,sleep was the same as that in the hypoxia control group at night. ③ The group of training of great exercise tolerance in hypoxia （Group C） （n=12）： Running in upgrade （15°） 80%-90% VO2 max , speed , exercise time and sleep were the same as that in the Group B . ④ Normoxic control group （Group D） （n=7）, the group of training of middling exercise tolerance in normoxic environment （Group E） （n=9） and the group of training of great exercise tolerance in normoxic environment （Group F） （n=8） . The exercise mode was the same as that in the corresponding hypoxia group. Rats rested in normoxic environment （0.21 volume fraction oxygen） at night. 9 weeks later, serum basic alkaline phosphatase（ALP） activity and serum tartrateresistant acid phosphatase （TRAP） of blood serum , urinary hydroxyproline （HOP） and creatinine（Cr） of stale were measured. RESULTS： All the 55 rats entered the stage of result analysis.① Activity of.serum ALP in the Group E or Group F was significantly higher than that in the Group D. Among the normoxic or hypoxia group, the activity

关 键 词：氧 运动(Exercise) 生长 碱性磷酸酶 羟脯氨酸 肌酐 

分 类 号：R681[医药卫生—骨科学]