鼠疫菌免疫胶体金快速检测方法的建立  

Development of an immunochromayography assay method for the detection of Yersinia pestis

作　　者：朱虹[1] 张春华[2] 檀华[1] 何君[1] 赵斌[2] 端青[1] 

机构地区：[1]军事医学科学院微生物流行病研究所微生物检验研究中心,北京100071 [2]中国疾病预防控制中心鼠疫布鲁氏菌病预防控制基地

出　　处：《中华流行病学杂志》2006年第3期253-255,共3页Chinese Journal of Epidemiology

摘　　要：目的利用免疫胶体金技术建立一种简便、快速并适用于基层人员使用的鼠疫菌抗原检测方法。方法将抗鼠疫菌F1抗原的抗体致敏硝酸纤维素膜,用于捕获鼠疫抗原,然后用免疫胶体金颗粒进行标记。结果应用鼠疫菌免疫胶体金快速检测法最低可检出不同鼠疫菌(EV和Evp株)1.56×105CFU/ml,检测鼠疫菌F1抗原最低可检出1ng/ml,与鼠疫反向间接血凝法的检测结果一致;并且不与假结核小肠结肠炎等耶尔森菌以及其他相关细菌发生交叉反应。结论鼠疫菌免疫胶体金快速检测法具有较好的特异性、敏感性和简便快速的特点。Objective To develop a method of imrnunochromatography assay（ICA） with sensitive, specific,rapid, simple and suitable for the detection of Yersinia pestis antigen at the local laboratories. Methods Colloidal gold labeled with the anti-Fl antibody of Yersinia pestis, was connnected with the anti-F1 antibody of Yersinia pestis to pyroxylin membrane and assembled them to the dipstick of ICA. Results Results showed that the rates of sensitivity for F1 antigen and Yersinia pestis were 1 ng/ml and 1.56 × 10^5 CFU/ml respectively. However, Yersinia pseudotuberculosis et al could not be detected by dipstick MICA. Conclusion The method of ICA appeared to be consistent to those of r-IHA with better specificity and sensitivity but was simple and rapid for the detection of Yersinia pestis and F1 antigen.

关 键 词：鼠疫菌 鼠疫菌F1抗原 胶体金 免疫层析 

分 类 号：R446.6[医药卫生—诊断学] R563.1[医药卫生—临床医学]