还原型尼克酰胺腺嘌呤二核苷酸脱氢酶（NADPH－d）组化方法操作步骤中各种参数的选择  

DETERMINATION OF OPTIMAL CONDITIONS FOR VARIOUS PARAMETERS IN THE PROTOCOL OF HISTOCHEMICAL TECHNIQUE ON REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (NADPH)-DIAPHORASE

作　　者：熊抗辉[1] 赵经纬[1] 王百忍[1] 

机构地区：[1]西安第四军医大学基础部解剖学教研室

出　　处：《神经解剖学杂志》1996年第1期79-82,共4页Chinese Journal of Neuroanatomy

摘　　要：为了提高还原型尼克酰胺腺嘌呤二核苷酸脱氢酶（ＮＡＤＰＨ－ｄ）组化方法的效果、稳定性和敏感性，本研究选用合适的操作条件，观察和比较了该方法操作步骤中各种参数改变对反应结果的影响。注意到操作过程中的各个环节和因素的变化均会不同程度地影响实验结果。对此法的固定、切片、反应和反应后处理过程等操作步骤的最佳条件提出了建议。The influences of changing various parameters in the protocols of histochemical reaction of NADPH-diaphorase to result were compared in the brains of 16 rats. It is noticed that multifarious factors in the histochernical protocols affected the reliability, stability and sensitivity of reaction. The optimal conditions in each step of the protocol including fixation, sectioning,reaction and post reaction treatment were suggested.

关 键 词：组织化学 方法技术 NADPH-D 神经解剖学 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学] R322.8[医药卫生—基础医学]