结直肠腺癌患者血清线粒体肌酸激酶检测的意义  

The value of serum mitochondrial creatine kinase determination in patient with colorectal adeno carcinoma

作　　者：顾鹏飞[1] 张建[1] 仲人前[1] 王爱华[1] 

机构地区：[1]第二军医大学附属长征医院实验诊断科全军临床免疫中心,上海200003

出　　处：《检验医学》2006年第2期129-131,共3页Laboratory Medicine

摘　　要：目的通过对结直肠腺癌患者血清线粒体肌酸激酶(M tCK)的检测,探讨其在结直肠腺癌诊断中的临床意义。方法选择手术治疗的结直肠腺癌患者107例和同期非肿瘤患者53例为研究对象。用电泳法检测血清M tCK,同时用化学发光免疫测定检测癌胚抗原(CEA),酶联免疫吸附试验(ELISA)检测糖抗原(CA)242。结果结直肠腺癌患者组中M tCK、CEA、CA242的阳性率分别为59.8%、55.1%和63.5%,非肿瘤对照组为3.7%、5.7%和3.7%。M tCK检测的敏感性和特异性分别为59.8%和96.2%,与CEA、CA242联合检测的敏感性和特异性分别为44.8%和100.0%。结论血清M tCK检测有助于结直肠腺癌的诊断,与CEA和CA242联合检测可显著提高对结直肠腺癌诊断的特异性。Objective To detect the level of mitochondrial creatine kinase（MtCK） in serum in patients with colorectal adeno carcinoma and evaluate its clinical value in diagnosis of colorectal adeno carcinoma. Methods Serum MtCK, carcinoembryonic antigen（CEA） and carbohydrate antigen（ CA）242 of 107 patients with eolorectal adeno carcinoma and 53 non-tumor patients by electrophoresis, chemiluminescence immunoassay and enzyme linked immunosorbent assay （ELISA） respectively. Results The positive rates of MtCK, CEA and CA242 in patient with colorectal adeno carcinoma were 59.8% ,55.1% and 63.5% respectively, and significantly higher than those positive rates patients in patients non-colorectal carcinoma patients, which were 3.7% ,5.7% and 3.7% respectively. The sensitivity and specifici- ty of MtCK were 59.8% and 96.2%. In combined test with CEA and CA242, when all of them were positive, the sensitivity changed to 44.8% and the specificity was 100.0%. of colorectal adeno carcinoma. The combined use of MtCK, Conclusions The serum MtCK can be useful for diagnosis CA242 and CEA can elevate the diagnostic specificity.

关 键 词：线粒体肌酸激酶 腺癌 癌胚抗原 糖抗原242 

分 类 号：Q555[生物学—生物化学]