银杏提取物和潘生丁对兔心肌缺血再灌注后iNOS基因转录和翻译的影响  

Effect of Ginkgo Biloba Extract and Dipyridamole on Transcri ption and Translation of Inducible NO Synthase in Rabbits after Myocardial Isc hemia-Reperfusion Injury

作　　者：宋庆江[1] 王韶华[1] 杨捷[2] 孙洁[3] 严泉剑[2] 朱妙章[4] 郭志坤[2] 陈志恒 

机构地区：[1]山东中医药大学附属淄博市中医院内科,山东255300 [2]新乡医学院组织工程实验室 [3]中国中医科学院中药所 [4]第四军医大学生理教研室 [5]河南陈贾医院

出　　处：《中国中西医结合杂志》2006年第3期240-243,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：35批博士后基金(No.2004035054);国家自然科学基金(No.30500471);河南省杰出人才创新基金(No.HNIFOS0621002300)

摘　　要：目的研究银杏提取物(Egb761)与潘生丁单用及联用对家兔心肌缺血再灌注诱导型一氧化氮合酶(i NOS)的影响。方法参照文献方法制备兔缺血再灌注模型,35只兔随机分为5组,每组7只,假手术组、模型组、潘生丁(0.8mg/kg)组、银杏(Egb76140mg/kg)组及联合组,于再灌注30min后分别静脉注射相应的药物,同时检测心肌再灌注晚期i NOS转录水平和翻译水平表达变化。结果每组动物均存活至实验结束。心肌i NOS转录水平表达分别是假手术组0、模型组157.11±17.73、潘生丁组202.6±21.84、银杏组356.13±24.18和联合组562.34±35.19,与模型组比较,各用药组心肌i NOS转录水平表达显著增加(P<0.01)。心肌缺血再灌注中i NOS蛋白翻译水平表达分别是假手术组34.24±15.78、模型组75.70±13.71、潘生丁组116.89±22.57、银杏组143.75±16.05和联合组195.09±22.25,与模型组比较,各用药组i NOS蛋白翻译水平表达均显著增加(P<0.05或P<0.01)。结论潘生丁与银杏提取物单用和联用均增加家兔心肌缺血再灌注晚期i NOS表达,联用增加更明显。Objective To investigate the effects of Egb761, an extract of ginkgo biloba , and dipyridamole on inducible NO synthase （iNOS） in rabbits after myocardial ischemia-reperfusion injury. Methods After being established into ischemia-reperfusion injury model, 35 rabbits were divided randomly into 5 groups： Group A （the sham group）, Group B （the model group）, Group C （treated with dipyridamole 0.8 mg/kg）, Group D （treated with Egb761, 40 mg/kg）,and Group E （treated with Egb761 40 mg/kg combined with dipyridamole 0.8 mg/kg）, all the medications were administered by intravenous injection 30 min after reperfusion. After administration, myocardial iNOS mRNA expression was detected by RT-PCR and western blot. Results Myocardial iNOS mRNA transcriptive expression in the 5 groups were A 0, B 157.11 ± 17.73, C 202.6 ± 21.84, D 356.13 ±24.18 and E 562.34 ± 35.19 respectively, showing significant difference between the treated groups and group B （P 〈0.01）. The translative expression of myocardial iNOS in the 5 groups were A 34.24 ± 15.78, B 75.70±13.71, C 116.89±22.57, D 143.75 ±16.05 and E 195.09 ±22.25 respectively, showing significant difference between the treated groups and group B as well （P 〈0.05, P 〈0.01）. Conclusion Both Egb761 and dipyridamole could increase myocardial iNOS expression in transcriptive and translative levels in rab bits after myocardial ischemia-reperfusion injury, and the combined treatment of them shows a more significant effect.

关 键 词：银杏提取物 潘生丁 再灌注损伤 诱导型一氧化氯合酶 

分 类 号：R96[医药卫生—药理学]