中国HIV-1感染疾病长期不进展者CCR2-64I、SDF1-3′A和CCR5Δ32的基因变异研究  

Variant genotyping of CCR2-64I,SDF1-3′A and CCR5Δ32 in HIV-1 infected Chinese long-term nonprogressors

作　　者：王树祥[1] 尚红[1] 韩晓旭[1] 张子宁[1] 王亚男[1] 张旻[1] 姜拥军[1] 刘静[1] 苏艳丽[1] 

机构地区：[1]中国医科大学附属第一医院艾滋病研究所,沈阳110001

出　　处：《中华实验和临床病毒学杂志》2006年第1期16-19,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金资助项目(30471548);国家十五科技攻关课题(2004BA719A12);辽宁省教育厅科技攻关计划(2012215112)

摘　　要：目的探讨CCR2-64I、SDF1-3′A、CCR5Δ32基因变异对中国HIV-1感染疾病长期不进展者(long-termnonprogressors,LTNPs)疾病进程的影响。方法收集17例中国HIV-1感染LTNPs和39例中国HIV-1感染疾病典型进展者(typical progressors,TPs)的抗凝全血标本,用全自动核酸提取仪提取基因组DNA,采用PCR/RFLP技术对CCR2、SDF1、CCR5基因进行检测分析。结果LTNP组的CCR2-64I、SDF1-3′A基因突变率分别为50%和62·5%,TP组的CCR2-64I和SDF1-3′A基因突变率分别为23·08%、33·33%,LTNP组CCR2-64I、SDF1-3′A基因突变率明显高于TP组(P<0·05);LTNP组有1例CCR5Δ32杂合突变,未发现纯合变异,而TP组未发现CCR5Δ32突变。结论CCR2-64I、SDF1-3′A和CCR5Δ32基因突变可能是中国HIV-1感染者疾病长期不进展的保护性因素之一。Objective To investigate variant genotyping of CCR2-641,SDF1-3＇A and CCR5Δ32 in HIV-1 infected Chinese Long-term nonprogressors and to study their association with disease progression. Methods The genotypes of CCR2-64I,SDF1-3＇A and CCR5Δ32 were detected by polymerase chain reaction/restriction fragment length polymorphism （PCR/RFLP） assay in seventeen HIV-1 infected Chinese Long-term nonpragressors （LTNPs） and thirty-nine Chinese typical progressors （TPs）. Results The frequency of CCR2-64I and SDF1-3＇Ain LTNPs are 50% and 62.5% ,higher than those （23.08%and 33.33% ） in TPs. Only one heterozygous CCR5 mutant was detected in LTNPs,and no CCR5 mutant in TPs. Conclusion Variant genotypiag of CCR2-64I, SDF1-3＇A and CCR5Δ32 may be protective factors for delaying disease progression in HIV-1 infected Chinese LTNPs.

关 键 词：HIV-1 受体 HIV 受体 趋化因子 

分 类 号：R512.91[医药卫生—内科学]