乙型肝炎患者特异性细胞免疫功能检测的初步研究  

Preliminary study on determination of specific cellular immunity of patients with hepatitis B

作　　者：赫兢[1] 辛绍杰[1] 沈宏辉[1] 杨建洋[1] 朱雷[1] 胡燕[1] 侯俊[1] 貌盼勇[1] 

机构地区：[1]解放军第三○二医院传染病研究所病毒研究室,北京100039

出　　处：《中华实验和临床病毒学杂志》2006年第1期33-35,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的通过酶联免疫斑点法检测乙型肝炎(乙肝)患者特异性细胞免疫功能,初步研究各型乙肝患者特异性细胞免疫功能的差异。方法选择急、慢性乙肝、肝炎肝硬化(乙型)、乙肝病毒(HBV)既往感染,接种乙肝疫苗后血清乙肝病毒学标志中仅表面抗体阳性及未接种过乙肝疫苗、未感染过HBV、血清HBV标志全部阴性者共6组研究对象,每组4例,采用酶联免疫斑点法(ELISPOT)检测其外周血单个核细胞中γ-干扰素分泌细胞的数量。结果急性乙肝患者、慢性乙肝患者及急性乙肝患者、肝炎肝硬化患者外周血单个核细胞γ-干扰素分泌细胞数量明显不同(P=0·0209及P=0·0211)。结论通过ELISPOT检测乙肝患者外周血单个核细胞γ-干扰素分泌细胞的数量可以了解乙肝患者特异性细胞免疫功能。急性乙肝患者特异性细胞免疫功能明显强于慢性乙肝患者及肝炎肝硬化患者。Objective To evaluate an enzyme linked immunospot （ELISPOT） method for testing the specific cellular immunity of patients with hepatitis B and preliminarily investigate into the difference of cellular immunity in patients with various types of hepatitis B. Methods The patients with acute hepatitis B, chronic hepatitis B, liver cirrhosis, healthy persons with HBV vaccine immunization, healthy persons with past HBV infection and HBV naive persons were enrolled in this study. Their peripheral blood mononuelear cells were tested by ELISPOT to determine the number of T-interferon secreting cells. Results The number of T-interferon secreting cells was significantly different between the patients with acute hepatitis B and those with chronic hepatitis B, and between the patients with acute hepatitis B and those with liver cirrhosis （P = 0.0209 and P = 0.0211 ）. Conclusion The specific cellular immunity in the patients with hepatitis B could be evaluated by determining the number of T-interferon secreting ceils with the method of testing their peripheral blood mononuclear cells by ELISPOT. The specific cellular immunity was stronger in the patients with acute hepatitis B than in those with chronic hepatitis B and liver cirrhosis.

关 键 词：肝炎 乙型 免疫 细胞 酶联免疫斑点法 

分 类 号：R512.62[医药卫生—内科学] R446.61[医药卫生—临床医学]