2001年徐州地区暴发性病毒性脑膜炎病原的研究  

Etiology of aseptic meningitis prevalent in Xuzhou

作　　者：李显[1] 郭喜玲[1] 史智扬[1] 倪大新[1] 汪华[1] 

机构地区：[1]江苏省疾病预防控制中心微生物检验室,南京210009

出　　处：《中华实验和临床病毒学杂志》2006年第1期66-69,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的确定引起2001年江苏省徐州地区无菌性脑膜炎流行的病原体。方法组织培养法从患者脑脊液分离病毒,标准血清中和试验鉴定分离毒株;中和试验检测双份血清中和抗体效价;逆转录聚合酶链反应(RT-PCR)检测肠道病毒特异性基因片段。结果22份脑脊液中分离出4株柯萨奇B5型、2株柯萨奇B3型、1株艾可7型肠道病毒,分离阳性率31.8%。RT-PCR检测脑脊液21份,肠道病毒阳性11份,阳性率52.4%。19例双份血清中11例中和效价呈4倍以上增长或转阳,阳性率57.9%。结论此次江苏省徐州地区无菌性脑膜炎流行的病原体是以柯萨奇B5为主要血清型的肠道病毒。Objective To find the pathogenic agents of aseptic meningitis prevalent in Xuzhou of Jiangsu province in 2001. Methods The enterovirus （EV） was cultured from CSF of the patients and identified with antiserum by neutralization test. Neutralization titcr of antibody in paired sera from meningitis children was determined. EV RNA was detected by RT-PCR. Results Four strains of Coxsackievirus B5,2 strains of Coxsackievims B3 and 1 strain of Echovims 7 were isolated from 22 CSF specimens. The isolation rate of virius was 31.8% （7122） ,21 CSF were tested by RT-PCR, the positive rate of EV RNA was 52.4% （11/21）; 57.9% （11/19） of patients＇paired-sera had over 4 folds antibody rise or became sereconverted. Conclusion Enterovims was the pathogenic agent of aseptic meningitis prevalent in Xuzhou of Jiangsu province, the main serotype of the vires was Coxsackievims BS.

关 键 词：脑膜炎 无菌性 肠道病毒属 柯萨奇病毒感染 艾可病毒感染 

分 类 号：R373[医药卫生—病原生物学]