首乌藤药材质量标准的研究  

Study on Quality Standards for Caulis Polygoni Multiflori

作　　者：范宋玲[1] 孙冬梅[1] 王洛临[1] 罗文汇[1] 

机构地区：[1]广东省中医研究所,广东广州510095

出　　处：《中国实验方剂学杂志》2006年第3期7-9,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技攻关计划(No:2001BA701A38)

摘　　要：目的：建立首乌藤药材的质量标准。方法：采用TLC法对首乌藤药材进行定性鉴别；用高效液相色谱法测定2,3,5,4＇-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量。结果：在TLC色谱中首乌藤斑点清晰；2,3,5,4＇-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.0309～1.54μg范围呈良好的线性关系，r=0.99965：平均回收率为95．5％，RSD=3．2％。结论：所建立的方法可准确地进行定性、定量检测，可用于首乌藤药材的质量控制。Objective： To establish the quality standards for Caulis Polygoni Multiflori. Method： The TLC and HPLC methods were used for identifi-cation and quantitative determination of 2,3,5,4＇-Tetrahydroxystilbene-2-O-β-D-glucoside. Result： Caulis polygoni multiflori could be identified by TLC. 2,3,5,4＇-Tetrahydroxystilbene-2-O-β-D-glucoside showed a good linear relationship at a range of 0.0309～1.54μg, r = 0.99965. The average recovery was 95.5%, and RSD was 3.2%. Conclusion： The methods can be used for the quality control of Caulis Polygoni Muhiflori.

关 键 词：首乌藤 高效液相色谱法 2 3 5 4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 

分 类 号：R284.1[医药卫生—中药学]