水稻线粒体双链RNA的研究  

A PRELIMINARY STUDY ON MITOCHONDRIAL DOUBLE STRANDED RNA IN RICE

作　　者：郭宝太[1] 姜国勇[1] 王京兆[2] 王斌[2] 

机构地区：[1]莱阳农学院农学系 [2]中国科学院遗传所

出　　处：《莱阳农学院学报》1996年第2期79-83,共5页Journal of Laiyang Agricultural College

基　　金：洛克菲勒基金

摘　　要：电泳图谱上，由水稻ＢＴ型不育系４－７１Ａ及其保持系４－７１Ｂ黄化芽提取的线粒体核酸在线粒体ＤＮＡ主带附近都有一条额外的核酸带，称之为Ｍ带。ＲＮａｓｅＡ与ＤＮａｓｅⅠ试验表明Ｍ带是双链ＲＮＡ。ＤＮＡ－ＲＮＡ分子杂交证明Ｍ带与核ＤＮＡ有同源性，与线粒体ＤＮＡ无同源性。Ｍ带在Ａ、Ｂ两系中对等存在，与细胞质雄性不育无直接关系；Ｍ带存在于线粒体中而与核ＤＮＡ有同源性。Double stranded RNAs(dsRNAs) have been detected in cytoplasmic male sterile line 4 71A of BT type rice and it's maintainer line 4 71B. The double strandedness of the RNA molecules was characterized by DNase Ⅰ and RNaseA treatments. The dsRNAs are associated with rice mitochondria, but have homology to the 4 71 genome. Both 4 71A and 4 71B contain dsRNA, it appears that the presence of the dsRNA in male sterile line is not associated with it's male sterility . The dsRNAs may be responsible for correlation between nuclear genome and mitochondrial genome.

关 键 词：双链RNA 线粒体 水稻 

分 类 号：S511.032[农业科学—作物学]