独一味水提取物止血作用及其机理的初步研究  

Study on the Hemostatic Effect of Extracts from Herb Lamiophlomis rotata and Its Mechanism

作　　者：沈涛[1] 贾正平[2] 李茂星[2] 张汝学[2] 张华欣[3] 

机构地区：[1]兰州大学药学院,兰州730000 [2]兰州军区总医院药材科,兰州730050 [3]兰州军区总医院检验科,兰州730050

出　　处：《中药新药与临床药理》2006年第2期93-96,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：兰州军区医药卫生科研基金资助项目(LXH-200409);甘肃省科技攻关项目(2GS054-A43-014-14)

摘　　要：目的研究藏药独一味水提取物(HLRE)的止血、促凝血作用及其机理。方法小鼠灌胃给予HLRE高、中、低剂量,3天后测定止血时间(BT)和凝血时间(CT),同时进行血小板计数(PLC);大鼠不同剂量口服给药14d后,颈总动脉采血,测定凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(FIB)含量以及肝功能和血脂等指标。结果HLRE2g/kg剂量组与1g/kg剂量组与生理盐水组比,出血时间和凝血时间都显著缩短,但各给药组血小板计数无显著性差异。PT、APTT无差异;TT明显缩短;FIB、Alb(白蛋白)显著增加;转氨酶、血脂有降低趋势。结论HLRE具有较好的止血、促凝血作用,推测其可能是通过增加纤维蛋白原(FIB)的含量,缩短大鼠凝血酶时间(TT)来发挥上述作用的。Objective To study the effect of Tibet medicine of Herba Lamiophlomis rotata on promoting hemostasis and blood coagulation and to explore its mechanism. Methods High dose（2 g/kg）, middle dose（1 g/kg） and low dose （0. 5 g/kg） of the aqueous extract from Herba Lamiophlomis rotata （HLRE） were given to mice by gastric gavage for 3 days, and then bleeding time （BT）, clotting time（CT） and platelet count （PLC） were determined. Different doses of HLRE （3, 1.5, 0.75 g/kg） were administered by gastric gavage to rats for 14 days, and then blood samples were collected from common carotid artery for the determination of prothrombin time （PT）, activated partial thromboplastin time （AVIT）, thrombin time （TT） , fibrinogen （FIB） , hepatic function and blood lipid indexes. Results Compared to the blank control group, the bleeding time and clotting time were obviously shortened in 2g/kg HLRE and 1g/kg HLRE groups and positive control group, but the difference of PLC was insignificant. PT and AVIT values in all of the treatment groups did not differ from those in the blank control group. However, TT values were obviously shortened in 3 g/kg HLRE group, FIB and Albumen （A1b） values increased, the aminotransferase and blood lipid values tended to decrease. Conclusion The aqueous extract from Herba Lamiophlomis rotata has an effect on promoting hemostasis and blood coagulation , and its mechanisms may be related to the increase of FIB and Alb contents and the shortening of TT value.

关 键 词：独一味水提物 止血 凝血酶时间 纤维蛋白原 白蛋白 

分 类 号：R285.5[医药卫生—中药学]