解脲支原体及其不同血清型对尿路清的药敏研究  

Susceptibilities of Ureaplasma Urealyticum and Its Different Serotypes to Niaoluqing Decoction

作　　者：江光明[1] 陆原 陈达灿[3] 榻国维[3] 

机构地区：[1]深圳市罗湖区人民医院皮肤科,深圳518001 [2]深圳市第六人民医院,深圳518052 [3]广州中医药大学第二临床医学院,广州510120

出　　处：《中药新药与临床药理》2006年第2期102-104,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省科委重点科技攻关项目(970101)

摘　　要：目的探索尿路清对解脲支原体(Uu)感染的体外抗菌药效,为中医药治疗本病提供依据。方法63个Uu临床分离株采用肉汤稀释法判定耐红霉素和四环素(MIC≥16μg/mL,参照美国国家临床实验室标准委员会1995年标准);以Uu1～14型抗血清,采用MIT法作血清分型后,经3次传代成纯培养物后接种于96孔板小孔中,药液倍比稀释成250mg/mL～0.48mg/mL,37℃培养,加药组72h后仍不出现培养基由黄色变为红色的最小药物浓度为该药的最小抑菌浓度(MIC)。结果尿路清对63株Uu临床株MIC范围为0.48～15.63mg/mL,MIC50≤1.95mg/mL,MIC90≤3.91mg/mL。Uu临床株对尿路清的敏感性比对四环素高得多(χ2=18.38,P<0.01);Uu临床株对尿路清的敏感性与对红霉素的敏感性之间无明显联系(χ2=0.29,P>0.05)。14种和未定型Uu血清型别中,除11,1,2,3型对尿路清的敏感性稍差(极少数菌株MIC≥7.81mg/mL),其余血清型别对尿路清的敏感性均较高(MIC≤3.91mg/mL)。结论中药尿路清对63株不同血清型、耐药(四环素、红霉素)菌株Uu显示出了较强的体外抑菌作用。Objective To study the in- vitro antibacterial activity of Niaoluqing Decoction on Ureaplasma Urealyticum （Uu） . Methods Sixty - three clinical strains of Uu were identified to determine their serology and antibiotic susceptibilities by the metabolic inhibition test（MIT）. These strains were subcultured for 3 generations and then cultured with Niaoluqing Decoction with a series of concentrations from 250 mg/mL to 0. 48 mg/mL. The minimal inhibitory concentration （MIC） was determined when the color of culture medium did not change from yellow to red in 72 h. Results For 63 clinical strains of Uu, the range of MIC of Niaoluqing Decoction was from 0. 48 mg/mL to 15.63 mg/mL, MIC50 ≤〈 1.95mg/mL, MIC90 ≤ 3.91 mg/mL. Clinical strains of Uu had a higher sensitive to Niaoluqing Decoction than to tetracycline （x^2= 18.38, P 〈 0. 01）, but the difference of antibiotic susceptibilities was insignificant between erythromycin and Niaoluqing Decoction （x^2 = 1.24, P 〉 0.05）. Clinical strains of Uu including all of its serotypes had a higher antibiotic susceptibility to Niaoluqing Decoction （MIC ≤ 3.91 mg/mL） except for serotype 11, 1, 2 in this experiment contains （MIC ≥ 7.81 mg/mL）. Conclusion Niaoluqing Decoction showed a significant in - vitro antibacterial activity on clinical Uu strains with different serotypes.

关 键 词：尿路清 解脲支原体 药敏试验 四环素 红霉素 血清型 

分 类 号：R285.5[医药卫生—中药学]