结缔组织生长因子与转化生长因子β1在肝纤维化组织中的表达  

The expression of connective tissue growth factor and transforming growth factor β1 in hepatic tissue and the correlation between them during hepatic fibrosis

作　　者：陆翠华[1] 华国平[1] 王华[1] 顾美珍[1] 倪润洲[1] 黄介飞[1] 

机构地区：[1]南通大学附属医院消化内科,南通226001

出　　处：《南通大学学报（医学版）》2006年第2期91-93,共3页Journal of Nantong University(Medical sciences)

摘　　要：目的:研究结缔组织生长因子与转化生长因子β1在肝纤维化组织中的表达及其相关性。方法:用CCl4皮下注射法制备肝纤维化模型,用免疫组化方法动态检测结缔组织生长因子(CTGF)、转化生长因子β1(TGFβ1)、I、III型胶原在肝纤维化组织中的表达。将免疫组化结果行图像扫描半定量分析后进行统计学分析。结果:正常对照组CTGF不表达,TGFβ1微量表达,I、III型胶原主要分布在门静脉、中央静脉内皮下以及Disse间隙。随着CCl4注射时间的延长,肝纤维化程度的加深,上述4种蛋白质在肝纤维化组织中的表达逐渐增加,且CTGF与TGFβ1之间存在着显著的相关性。结论:结缔组织生长因子与转化生长因子β1相伴表达,且与肝纤维化的发生密切相关,可作为肝纤维化治疗的新靶点。Objective：To invest the expression of connective tissue growth factor （CTGF） and transforming growth factor β1 （TGFβ1） in hepatic tissue and the correlation between them during hepatic fibrosis. Methods ：The model of hepatic fibrosis in rats was induced by injection of carbon tetrachloride （CCl4）. CTGF, TGFβ1, Collagen Ⅰ and Ⅲ in hepatic tissues of these rats were detected with immunohistochemistry. The correlation between CTGF and TGFβ1 was analyzed after the results of immunohistochemistry were semi- quantified. Results：CTGF was not detected in normal controls. TGFβ1 was detected little in normal controls. Collagen Ⅰ , Ⅲ were distributed in portal tracts, endothelium in central vein and space of Disse. 2,4 and 6 weeks after CCl4 injection, the above 4 proteins were further enhanced, and CTGF had a significant correlation with TGFβ1 （P 〈 0.05）. Conclusion：The CTGF is overexpressed during the procedure of hepatic fibrosis, and has significant correlation with TGFβ1, suggesting that CTGF may provide a new target for the treatment of hepatic fibrosis.

关 键 词：肝纤维化 结缔组织生长因子 转化生长因子Β1 

分 类 号：R692[医药卫生—泌尿科学] R575.2[医药卫生—外科学]