饮水中微量碘的化学发光测定  

Detection of Iodine in Drinking Water by Chemiluminescent Assay

作　　者：徐顺清[1] 周宜开[1] 郝巧玲[1] 舒柏华[1] 

机构地区：[1]同济医科大学环境医学研究所

出　　处：《中国卫生检验杂志》1996年第4期187-189,共3页Chinese Journal of Health Laboratory Technology

摘　　要：Ｉ＿２能与鲁米诺发生化学发光反应，以表面活性剂ＣＴＡＣ为介质能增强发光反应的强度，发光强度与Ｉ２浓度成正比。本文利用这一原理建立了一种高灵敏度的碘测定方法，其检测下限为０．２ｎｇ／ｍｌ，同批样本重复测量的变异系数为２．２０％。该法操作十分简便。Iodine causes chemiluminescence upon mixing with the CTAC reserved micelles solution of luminol. the CTAC can enhance chemiluminescence signal of iodine-luminol reaction.The chemiluminescence intensity is proportional to iodine concentration. The detection limit of iodine by chemiluminescence assy was 0.2ng/ml The Precision and accuracy of chemiluminescent assy in quite satisfactory, the relative standard deviation of reproducibility is 2.20%, The procedure of assay is very simple,after iodide was oxidated to 12 by H2SO4 and K2Cr2O7, I2 was extracted by cyclohexane, then mixed the cyclohexane solution of I2 with reserved micelles solution of luminol,the chemiluminescence signal was recorded by luminometer at once.

关 键 词：水质分析 碘 光学发光 

分 类 号：R123.1[医药卫生—环境卫生学]