氯氮平与蛋白质相互作用的研究  

Spectrometric Study on the Interaction between Clozapine and Bovine Serum Albumin

作　　者：关洪亮[1] 原华平[1] 潘祖亭[1] 

机构地区：[1]武汉大学化学与分子科学学院,湖北武汉430072

出　　处：《分析测试学报》2006年第2期25-28,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20275028)

摘　　要：用荧光光谱法和紫外-可见光谱法研究了在模拟人体生理条件下,氯氮平和牛血清蛋白(BSA)结合反应的特征,发现氯氮平对BSA有较强的荧光猝灭作用,且氯氮平的紫外吸收光谱和BSA的荧光光谱有一定程度的重叠,由此可得出其作用距离和结合过程的基本热力学参数。The binding characteristics of clozapine（CP） and bovine serum albumin（BSA） in aqueous solution were studies with fluorescence and absorption spectrometric methods. The results showed that CP had a powerful ability to quench the BSA fluorescence via nonradiative energy transfer mechanism. The fluorescence quenching data were analyzed according to Scatchard equation and double-reciprocal equation. The reaction distance and the thermodynamic parameters were obtained.

关 键 词：氯氮平 牛血清白蛋白 荧光光谱法 吸收光谱法 

分 类 号：O657.32[理学—分析化学] Q51[理学—化学]