北京、广州人群的高脂血清对单核细胞（U937）结合高密度脂蛋白的影响  

The Effects of Hypercholesterolemia from Beijing and Guangzhou on Monocyte(U937) Binding to High Density Lipoprotein

作　　者：梁平[1] 周梦玲[1] 申萍[1] 夏人仪[1] 佘铭鹏[1] 

机构地区：[1]中国医学科学院基础医学研究所,中国协和医科大学基础医学院病理室

出　　处：《中国动脉硬化杂志》1996年第1期1-4,共4页Chinese Journal of Arteriosclerosis

基　　金：国家"八.五"攻关课题

摘　　要：从北京、广州现场的血脂普查中选出高脂血清，分别作用于体外培养的单核细胞（Ｕ９３７），然后检测Ｕ９３７细胞对高密度脂蛋白的结合率。结果显示经北京高脂血清作用后的Ｕ９３７细胞对高密度脂蛋白的结合率显著增加，高密度脂蛋白中的载脂蛋白Ｅ对结合过程无明显影响；广州高脂血清对Ｕ９３７细胞无明显刺激作用，与正常血脂的作用相近。提示北京人群的高血脂可刺激单核细胞摄取脂质，广州高血脂无此作用。北京与广州人群的高血脂可能存在血脂组成与结构性质上的差异，进而影响动脉粥样硬化的发病率。The sera of hypercholesterolemia (HC)from people of Beijing and Guangzhou were incubated with U937 cells respectively for 40 hours,then binding rate between U937 cell and fluorescencelabeled high density lipoprotein(HDL) were detected on cell sorter. The result showed that the binding rate of cells treated with HC from Beijing was significantly higher(2～20 fold) than that from Guangzhou, of which the binding rate of cells was similar to control cells(treated with normal lipid sera). Apolipoprotein E(Apo E)and its receptor pathway did not play an important role in the binding process,HDL3 is major subclass of HDL which bound to U937 cells,These findings indicated that HC from Beijing has a role to stimulate monocyte cell to take lipids, while HC from Guangzhou doesn't. The difference should exist in plasma component between the masses of Beijing and Guangzhou with HC, which may contribute to high incidence of coronary heart disease in Beijing compared with that in Guangzhou.

关 键 词：高血脂 单核细胞 高密度脂蛋白 动脉粥样硬化 

分 类 号：R543.502[医药卫生—心血管疾病]