辛芷鼻敏胶囊对大鼠变应性鼻炎脾脏组织核转录因子-κB和白介素-5表达的影响及相关性  

Effects and Correlationship of Xinzhi Bimin Capsule on Expression of NF-κB and IL-5 in the Spleen Experimental Allergic Rhinitis in Rat

作　　者：包晓铃[1] 高丽[1] 刘俊昌[1] 刘江[1] 姜孝芳[1] 赵孟[1] 

机构地区：[1]新疆医科大学基础医学院,新疆乌鲁木齐830054

出　　处：《时珍国医国药》2006年第3期309-311,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金资助项目(No.30560144)

摘　　要：目的探讨辛芷鼻敏胶囊对实验性大鼠变应性鼻炎(A llergic rh in itis,AR)脾脏组织核转录因子-κB(Nuc lear factorkappa B,NFκ-B)和白介素-5(Interleuk in-5,IL-5)表达及相互关系的作用,明确药效机理。方法通过造模的大鼠灌服辛芷鼻敏胶囊及与同类药物对照,用免疫组化Evision方法半定量检测脾脏组织NFκ-B(亚单位P65)的活性细胞表达及双抗体夹心酶联免疫吸附技术(Enzym e labeled immunosorbent assay,ELISA)定量测定IL-5的表达。结果实验药物组的NF-κBP65的阳性细胞比例和IL-5含量明显低于模型组及阳性对照组(P<0.05);且NFκ-BP65的活性细胞比例与IL-5的表达呈正相关(r=0.935 5、P<0.05)。结论辛芷鼻敏胶囊可能通过降低核转录因子-κB活性,抑制大鼠AR发病过程中IL-5的表达,进而减少嗜酸粒细胞(Eosinoph il,EOS)的浸润而控制症状。Objective To study the effect and correlationship of Xinzhi Bimin capsule on expression of NF-κB and IL-5 in the spleen of experimental allergic rhinitis（AR） in rat,and identify the mechanism of pharmacodynamic action. Methods The expression of positive cell of NF-κB was detected in semiquantity by immunochemistry and the expression of IL-5 was detected in quanti- ty by ELISA,which were compared with the different model rat established in AR and the normal control group. Results The expression of the proportion of positive cell to NF-κB and IL-5 were lower in the experimental group than the model group （ P 〈 0.05） and the positive control group except the normal control group, moreover, the level of IL-5 in the experimental group was positively correlated to the proportion of positive cell to NF-κB. Conclusion The result suggests that the capsule of Xinzhi Bimin can reduce the infiltration of EOS and treat the symptom of AR in rat by down-regulating the activation of NF-κB,inhibiting the ex- pression of cytokines of Th2 （ such as IL-5）.

关 键 词：辛芷鼻敏胶囊 组织核转录因子-κB 白介素-5 双抗体夹心酶联免疫吸附技术 免疫组织化学 

分 类 号：R285.5[医药卫生—中药学]