地塞米松对血管平滑肌细胞增殖及一氧化氮合成酶基因表达的影响  

Effects of Dexamethasone on the Proliferation of Vascular Smooth Muscle Cells and the Expression of Nitric Oxide Synthase Gene

作　　者：乔亚明[1] 温进坤[1] 

机构地区：[1]河北医科大学基础医学研究所生物化学研究室

出　　处：《中国动脉硬化杂志》1996年第1期8-11,共4页Chinese Journal of Arteriosclerosis

基　　金：河北省自然科学基金

摘　　要：用３Ｈ-胸腺嘧啶核苷掺入实验及细胞计数检查地塞米松对体外培养的大鼠血管平滑肌细胞ＤＮＡ合成及细胞增殖的影响；经Ｎｏｒｔｈｅｒｎ印迹分析和亚硝酸盐含量测定观察一氧化氮合成酶基因表达及一氧化氮合成酶活性的变化。结果是，地塞米松显著抑制血管平滑肌细胞ＤＮＡ合成及分裂增殖，其抑制强度与地塞米松剂量和细胞被处理的时间呈正相关。地塞米松显著抑制肿后坏死因子α对血管平滑肌细胞一氧化氮合成酶表达的诱导作用，降低培养液内亚硝酸盐含量。证实地塞米松对血管平滑肌细胞ＤＮＡ合成及细胞增殖的抑制作用不是通过增加一氧化氮含量来实现的。In this study, the effects of dexamethasone(Dex) on the DNA ynthesis and proliferation of vascular smooth muscle cells(VSMC)were studied by 3H-thymidine(3H-TdR) incorporation and counting cell number. With Northern blot analysis and measuring the amount of nitrite, we observed the expression of inducible nitric oxide synthase (iNOS)gene in VSMC of control and treated by tumor necrosis factorα(TNF-α),TNF-α+Dex respectively.It was found that the DNA synthesis and proliferation of VSMCwere obviously inhibited by dexamethasone,the inhibitory rate was proportional to the dose and the duration of dexamethasone treatment.The results ofNorthern biotting and quantity of nitrite in culturemedium showed that dexamethasone obviously inhibited the expression of iNOS gene and nitric oxide (NO)production in VSMC induced with TNF-α. These results indicate it is not by increasing the NO production that dexamethasone inhibits the DNA synthesis and proliferation of VSMC.

关 键 词：地塞米松 血管平滑肌细胞 一氧化氮合成酶 药理 

分 类 号：R977.11[医药卫生—药品] R965[医药卫生—药学]