高耐汞菌株的筛选及其生物学特性研究  

Study on the Isolation and Bio-characteristics of High Mercury-resistant Bacteria

作　　者：阳菊华[1] 詹丽钦[1] 陈凌[2] 林宇岚[1] 包幼迪[1] 陈贻锴[1] 

机构地区：[1]福建医科大学医药生物工程中心,福州350004 [2]福建医科大学附属协和医院,福州350001

出　　处：《海峡预防医学杂志》2006年第1期5-7,共3页Strait Journal of Preventive Medicine

基　　金：福建省自然科学基金(C0510009);福建医科大学科学发展基金(FJGXY04020)

摘　　要：[目的]从汞污染土壤中寻找高耐汞菌。[方法]用HgCl2选择性培养基，分离和筛选出耐汞菌，测定苴耐Hg^2＋和耐药性水平，检测耐酸碱能力和还原Hg^2＋能力，分析遗传背景。[结果]共筛出7株耐汞菌，形态学观察和生化鉴定分属假单胞菌或枯草杆菌。其中假单胞菌CHY-7菌株耐药性较少，耐Hg^2＋水平高达100mg／L，营养要求低，耐酸碱范围广（pH5．0～10．0）；16S rRNA基因分类鉴定为恶臭假单胞菌；PCR和DNA测序证实该菌株含merA基因，位于细菌染色体上；在Hg^2＋为5～50mg／L时，48h后还原Hg^2＋为Hg^0的效率为94．3％到82．8％。[结论]该菌株有望应用于工业汞污水的处理和汞污染土壤及水体的修复。[Objective] To isolate high mercury-resistant bacterial strains （MRB） from mercury-polluted soil. [Methods] The MRB was isolated through HgCl2-selected media. The level of mercury-resistance, antibiotic resistance, pH-toleration, mercury chloride removal abilities and genetic background were analyzed. [Results] 7 MRBs were isolated and identified as Pseudomonas sp. or Bacillus subtilis. Among them, the isolate CHY-7 was less resistance to antibiotics and the Hg^2＋ tolerated reached to 100 mg/L. The CHY-7 was able to grow in low nutritional conditions, and possessed a remarkable ability to adapt to pH 5.0-10. 0. The genetic analysis showed that CHY-7 was identified as Pseudomonas purida by partical 16S ribosomal DNA sequencing and confirmed the presence of merA gene which was determined and located chromosomally by performing specific PCR. The CHY-7 reduced Hg^2＋ to Hg^0 was about 94.3%-82.8% （containing Hg^2＋ 5 50 mg/L in a 48 h）. [Conclusion] The isolate CHY-7 is a potential application prospect in bioremediation of mercury contaminated soil and water.

关 键 词：土壤卫生 汞污染 耐汞细菌 merA基因 生物修复 

分 类 号：R124[医药卫生—环境卫生学]