奶牛隐性乳房炎的主要病原菌的PCR鉴定  

Identification of Major Pathogens in Bovine Subclinic Mastitis by PCR

作　　者：吴润[1] 郝保青[1] 农向[1] 严丹红[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《中国牛业科学》2006年第2期12-14,17,共4页China Cattle Science

基　　金：四川省自然科学基金资助(项目号:04JY029-006-5)

摘　　要：基于细菌16 S rRNA和(或)23 S rRNA序列设计引物,利用PCR的方法鉴定出了奶牛隐性乳房炎的主要病原菌,包括金色葡萄球菌、大肠杆菌和无乳链球菌,避免了繁琐的培养过程,表明PCR技术是细菌鉴定非常有效、特异性高而又快速的方法,对隐乳的诊断很有价值。Primers were designed based on the DNA sequences of bacterium 16 S rRNA and （or） 23 S rRNA. This article presented a PCR detecting the major pathogens in subclinic mastiffs, including Staphylococcus aoreus ,Escherichia coli, Streptococcus agalactiae. The method avoided the redundant cell culturing process, therefore, the result showed that PCR technology is an effective, specific and rapid molecular tool to identify bacteria, and also a valuable method to diagnose bovine subclinic mastiffs.

关 键 词：隐性乳房炎 病原菌 PCR 

分 类 号：S823[农业科学—畜牧学]