不同受精方法对南阳牛卵母细胞体外受精的影响  

The Effect of Different Fertilization Methods on IVF of Oocytes in Nanyang Cattle

作　　者：胡文举[1] 王新庄 张英汉[1] 王居强 王涛 李金辉[2] 丁向彬[2] 郑培栋[2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]河南省肉牛工程技术研究中心,河南郑州450003 [3]郑州市奶牛胚胎工程技术研究中心,河南郑州450003

出　　处：《中国牛业科学》2006年第2期15-17,共3页China Cattle Science

基　　金：河南省杰出人才创新基金(0421002100);"十五"国家重大专项

摘　　要：本研究探讨了用直接离心法、上浮法和perco ll密度梯度离心法等3种方法处理精子对南阳牛体外受精效果的影响。结果表明3种方法处理的精子卵裂率差异不显著(84.2%vs84.5%vs83.6%),但是桑囊胚率perco ll密度梯度离心法和上浮法显著高于直接离心法(17.1%、16.9%vs13.8%)。同时比较了微滴法和微细管法对体外受精效果的影响,表明两种方法受精后的卵裂率差异不显著(84.1%vs81.1%),桑囊胚率微细管法显著高于微滴法(17.8%vs13.4%)。This research observed the effects of direct centrifugation, swimming-up, percoll density gradient centrifugation treated sperm on IVF in Nanyang cattle. The cleavage rates of sperm in the three treatments （84. 2%, 84. 5%, and 83.6%, respectively） were similar （P〉 0. 05）, but the development rates of morula and blastocyst in swimming-up and percoll density gradient centrifugation group （17. 1%, 16. 9%） were greater （P〈 0. 05） than that of direct centrifugation （13. 8%）. Meantime, comparison of the effect of drop and capillary methods on IVF indicated that the cleavage rates in the two treatment had no significant difference （P 〉 0. 05）, but the development rates of morula and blastocyst of capillary method were significantly higher than that of drop method （17.8%, 13.4%）.

关 键 词：南阳牛 卵母细胞 体外成熟 体外受精 卵裂率 

分 类 号：S823.4[农业科学—畜牧学]