黄瓜花叶病毒卫星RNA XJs1侵染性cDNA克隆构建及其生物学功能初步研究  

Construction of infectious cDNA clone of Cucumber mosaic virus satellite RNA XJs1 and preliminary study on its biological function

作　　者：席德慧[1] 林宏辉[1] 向本春[2] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064 [2]石河子大学农学院植物保护系,石河子832003

出　　处：《微生物学报》2006年第2期219-222,共4页Acta Microbiologica Sinica

基　　金：教育部博士点基金(20040610015);教育部优秀人才支持计划项目;四川省科技厅基金(04ZQ026-036);成都市科技攻关项目(05HJSW117)~~

摘　　要：利用RT_PCR的方法,获得了黄瓜花叶病毒卫星RNA XJs1的全长侵染性cDNA克隆pMSC20。序列分析显示,XJs1全长384nt(GenBank登录号:DQ070748),比较XJs1与具有代表性的CMV卫星RNA的序列结构表明,在XJs1核苷酸序列的325nt^350nt间,具有典型的坏死型卫星RNA保守序列。通过体外转录,将XJs1与不含卫星RNA的辅助病毒分离物CMV_AH组合接种普通烟和心叶烟并进行检测。初步研究结果表明,XJs1为一致弱卫星RNA。Cucumber mosaic virus （CMV） sugar beet isolate caused yellow mosaic, leaf distortion, crinkle and stunt symptoms on sugar beet in nature. It exhibited some special biological properties with narrower host range and had no symptom on Nicotiana glutinosa L. and Nicotiana tobacum L. cv. NC-89. A new satellite RNA, XJs1 was found to be associated with the helper virus, In order to know the cause of the special pathogenicity of the CMV isolate, Full-length infectious cDNA clone of CMV satellite RNA XJsl, pMSC20, was constructed by reverse transcriptase-polymerase chain reaction （RT-PCR）. Sequence analysis showed that the satellite RNA consists of 384 nucleotides （nt） （GenBank accession number; DQ070748）. Compared the nucleotide sequence of satellite RNA XJs1 with those of other representative CMV satellite RNAs displayed that it contains typical necrogenic consensus sequence block from positions 325 to 350, and shared 73.27% - 91.93% nucleotide sequence identity with some published CMV satellite RNAs, By in vitro transcription, satellite RNA XJs1 was inoculated on Nicotiana tabacum and Nicotiana glutinosa together with CMV-AH, a CMV isolate without satellite RNA. The results showed that satellite RNA XJs1 could attenuate symptoms on Nicotiana tabacum and Nicotiana glutinosa induced by CMV-AH. Detection by RT-PCR and Northern blot hybridization revealed XJs1 obtained replication in the above two host plants, showing the pathogenicity changes of CMV-AH on Nicotiana tabacum and Nicotiana glutinosa were induced by co-infecting with satellite RNA XJs1. These results indicated that XJs1 is probably an attenuate satellite RNA. The relationship between helper virus, satellite RNA and host plants is discussed.

关 键 词：黄瓜花叶病毒 卫星RNA 侵染性eDNA克隆 生物学功能 

分 类 号：S432.41[农业科学—植物病理学]