嗜盐菌素HalC8基因簇克隆与分析  

Cloning and analysis of genes in the Halocin C8 gene cluster

作　　者：梅双双[1] 厉云[1] 陆秋鹤[1] 向华[1] 

机构地区：[1]中国科学院微生物研究所微生物资源国家重点实验室,北京100080

出　　处：《微生物学报》2006年第2期318-322,共5页Acta Microbiologica Sinica

基　　金：国家"973项目"(2004CB719603);国家"863计划"(2004AA626010);国家自然科学基金项目(30570029)~~

摘　　要：采用基因组部分文库及锚定PCR技术,克隆了嗜盐菌素HalC8编码基因及其上下游可能的相关基因共约9.3kb的DNA序列。序列分析表明已知序列至少含有6个ORF,包括上游编码跨膜蛋白的halU基因、编码可能的调节蛋白的halR基因,编码嗜盐菌素HalC8及其免疫蛋白HalⅠ的proC8基因、以及位于proC8基因下游的编码可能的转运蛋白的halT1,halT2和halT3基因。这是国际上首次对嗜盐菌素基因簇可能的相关基因的克隆。Halocin C8 （HalC8）, produced by a halophilic archaeon strain AS7092, is a gene-coded peptide microhalocin and has a wide inhibitory spectrum against the members of. haloarchaea. To investigate the mechanisms of the gene expression regulation, the peptide processing and transportation of this halocin, a 9.3kb DNA gene cluster containing the halocin C8 encoding gene （proC8） and other possible involved genes was cloned, by screening of a genomic library of AS7092 as well as anchoring PCR technique. Sequence analysis indicated that it contained at least six open reading frames, including halU, haiR, proCS, halT1, halT2 and halT3. The gene halU encodes a membrane-spanning protein HalU, but its function is unknown. The gene haiR encodes a putative regulator protein HAIR, its function was deduced to regulate the transcription of proC8 gene, which encodes the precursor for halocin C8. The genehalT1, halT2 and halT3 likely encode the transporters HAITI, HalT2 and HalT3, the functions of which were speculated to transport the halocin C8 out of the cellular membrane. This is the first report of gene cluster cloning for any halocin.

关 键 词：嗜盐菌紊Halocin C8 基因克隆 序列分析 基因功能 

分 类 号：Q78[生物学—分子生物学] Q933