北京地区住院急性呼吸道感染患儿的病毒病原检测分析  

Analysis on Viral Etiology of Inpatient Children with Acute Respiratory Infection in Beijing Area

作　　者：袁艺[1] 宋国维[2] 杜军保[1] 曹玲[2] 崔小岱[2] 钱渊[2] 朱春梅[2] 伏谨[2] 朱汝南[2] 邓洁[2] 王芳[2] 赵林清[2] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]首都儿科研究所,北京100020

出　　处：《实用儿科临床杂志》2006年第6期348-350,共3页Journal of Applied Clinical Pediatrics

基　　金："十五"科技攻关计划项目资助(2003BA712A11-10)

摘　　要：目的了解北京地区住院急性呼吸道感染(ARI)患儿的病毒病原情况。方法取1260例年龄14岁以下住院ARI患儿的鼻咽深部分泌物,用间接免疫荧光及病毒分离法检测呼吸道合胞病毒(RSV)、流感病毒A、B型、副流感病毒1、2、3型及腺病毒等7种常见呼吸道病毒。用逆转录聚合酶链反应(RT-PCR)法对其中490例患儿标本进行肠道病毒(EV)检测。结果1.ARI1260例中,43.33%检测到了病毒病原,7种常见呼吸道病毒检出率36.19%,RSV阳性率最高(23.97%),以冬春季为著,88.08%的RSV阳性为3岁以下小儿。2.EV阳性率16.33%。3.19例存在2种病毒混合感染,均出现在冬春季,16例为RSV并EV感染。4.入选的510例急性呼气性喘息患儿中,3岁以下RSV阳性率最高(43.20%),3岁以上EV阳性率最高(36.11%)。结论1.RSV是北京地区冬春季婴幼儿ARI的主要病原。2.冬春季EV可并RSV等其他呼吸道病毒感染。3.RSV及EV是引起小儿急性喘息性疾病的主要病毒病原。Objective To investigate the viral etiology of inpatient children with acute respiratory infection（ARI） in Beijing area. Methods The nasopharyngeal secretion samples from 1260 inpatient children less than 14 years old with ARI were investigated for respiratory syncycial virus（RSV） ,influenza virus A and B,parainfluenza virus type 1,2,3 and adenovirus by both the indirect immunofluorecence assay and virus isolation. Four hundred and ninety cases were examined for enterovirus by reverse transcription polymerase chain reaction（RT-PCR）.Results 1.Viral agent was detected in 43. 33% of all cases, with RSV（23.97%） the most frequent（especially in cases under 3 years old）. 2. Enterovirus（EV） positive rate was 16.33%. 3. Mixed viral infection was found in 19 cases, with 16 cases coinfected with RSV and EV. 4. Among 510 cases suffering from acute expiratory wheezing, RSV was most frequent （43.20 % ）in cases under 3 years old while EV was most frequent （36.11% ） in cases older than 3 years old. Conclusions 1. RSV is still the leading cause of ARI in infants and young children during winter and spring in Beijing. 2. EV may play an important role in ARI in children. 3. RSV and EV are the main causes of acute expiratory wheezing in children.

关 键 词：呼吸道合胞病毒 肠道病毒 急性呼吸道感染 逆转录聚合酶链反应 

分 类 号：R725.6[医药卫生—儿科]