玉竹多糖干预后衰老模型鼠抗氧化系统及免疫功能的变化  

Effect of polygonatum odoratum polysaccharide on anti-oxidation system and immunological function of senile mice

作　　者：单颖[1] 潘兴瑜[2] 姜东[3] 金艳书[1] 赵良中[1] 

机构地区：[1]锦州医学院免疫教研室,辽宁省锦州市121001 [2]中国医科大学免疫教研室,辽宁省沈阳市110031 [3]锦州医学院解剖教研室,辽宁省锦州市121001

出　　处：《中国临床康复》2006年第11期135-137,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察具有扶正固本,养阴润燥和滋补强壮功效的百合科黄精属植物药玉竹的提取物玉竹多糖对D-半乳糖致亚急性衰老模型小鼠血清中超氧化物歧化酶活性和丙二醛含量的抗氧化系统作用及免疫功能的影响,探讨玉竹多糖抗衰老的可能作用途径。方法:实验于2004-07/2005-05锦州医学院免疫教研室完成。①取昆明小鼠50只,随机分为5组:正常对照组、衰老模型组和低、中、高剂量玉竹多糖治疗组。除正常对照组外,其余各组每只颈背部皮下注射50g/LD-半乳糖0.5mL,连续6周。同时低、中、高剂量玉竹多糖治疗组分别腹腔注射0.5,1,2g/kg剂量的玉竹多糖(玉竹为百合科黄精属植物药,药用根茎,由锦州医学院免疫教研室采摘,经中国医科大学免疫教研室潘兴瑜教授乙醇回流脱脂、水提纯、浓缩、醇沉、除蛋白、离心、真空干燥等程序,得到玉竹多糖;玉竹产自锦州凌海市温滴楼乡岩井寺工区)溶液0.5mL,正常对照组和衰老模型组小鼠给予腹腔注射生理盐水0.5mL。②给药6周后,麻醉下将小鼠摘除眼球取血,常规分离血清,在4℃下3000r/min离心5min,取上清待测。用分光光度计检测小鼠血清中超氧化物歧化酶活性和丙二醛水平,并将小鼠称质量并剖取小鼠胸腺和脾脏,用电子天平称质量,以脏器湿质量(mg/g)表示小鼠脾指数和胸腺指数,用MTT法测定脾淋巴细胞转化指数,用免疫荧光法测定脾T淋巴细胞亚群,观察玉竹多糖对免疫功能的影响。③多组间样本均数比较采用方差分析。结果:昆明种小鼠50只均进入结果分析。①衰老模型组小鼠血清中超氧化物歧化酶活性明显低于正常对照组,丙二醛水平明显高于正常对照组(P<0.05)。高剂量玉竹多糖治疗组小鼠血清中超氧化物歧化酶活性明显高于衰老模型组,丙二醛水平明显低于衰老模型组(P<0.01,0.05);中、低剂量玉竹多糖治疗组与衰老模型组血清�AIM： To observe the effect of polygonatum odoratum polysaccharide, which has effects of strengthening the body resistance to consolidate the constitution, nourishing yin to moisten dryness and strengthening by means of tonics, on the anti-oxidation system and immunological function of superoxide dismutae （SOD） activity and malondialdehyde （MDA） content in mice with subacute senile induced by D-galactose, and explore the possible anti-aging action＇of polygonatum odoratum polysaccharide. METHODS： The experiment was completed in the Department of Immunology of Jinzhou Medical University between July 2004 and May 2005.①Fifty Kunming mice were randomly divided into five groups： control group, senile model group and 3 treatment groups with low, medium and high dose of polygonatum odoratum polysaccharide. The 50 g/L 0.5 mL Dgalactose were injected subcutaneously into the naps of mice except control group for six successive weeks; Meanwhile the mice of 3 treatment groups were injected intraperitoneally with 0.5 mL polygonatum odoratum polysaccharide solutions of 0.5 g/kg, 1 g/kg, 2 g/kg respectively （Polygonatum odoratum were from Linghai of Jinzhou city, and provided by the Department of Immunology, Jinzhou Medical University. Polygonatum odoratum polysaccharide were obtained by Professor Pan Xing-yu of Department of Immunology, China＇Medical University in the sequences of ethanol defathng, purification, concentration, protein-removal, centrifugation and vacuum dehydration）. The mice of control group and senile model group were injected intraperitoneally with 0.5 mL saline. ②Six weeks later, the eyeballs of mice were taken out at anesthesia stage; Serum was separated routinely and centrifuged at 3 000 r/minute for 5 minutes at 4 ℃. The upper serum was obtained for measurement. The SOD activity and MDA content in .the serum of mice were detected with spectrophotometer. Meanwhile, mice were weighted, and thymus and spleen were taken out to weight with electronic balance. Wet mass of internal

关 键 词：玉竹 多糖类 衰老 丙二醛/血液 超氧化物歧化酶/血液 

分 类 号：R161.7[医药卫生—公共卫生与预防医学]