三重PCR方法检测猪链球菌2型  

A Triple PCR for the Detection of Streptococcus suis Type 2

作　　者：薛素强[1] 范伟兴 洪洁心[3] 黄保续 蔡振鸿 郭霄峰[1] 

机构地区：[1]华南农业大学兽医微生物室,广州510642 [2]国家动物流行病学中心,青岛266032 [3]广东出入境检验检疫局动植物监管处,广州510623

出　　处：《中国动物检疫》2006年第4期22-24,共3页China Animal Health Inspection

摘　　要：根据发表文献,设计合成三对引物,建立三重PCR方法,分别扩增猪链球菌2型cps2J、epf,mrp基因,目标片断大小分别为:230bp、570bp、860bp。对三重PCR方法进行特异性、敏感性、重复性试验,结果显示该三重PCR方法特异性好,敏感性高,重复性好,能快速的检测猪链球菌2型。Three pairs of primers were designed and synthesized according to the published literatures and a triple PCR was developed to amplify CPS2J, epf and mrp gene sequences of Streptococus suis Type 2, with the target sequeuces of 230bp, 570bp and 860bp.The triple PCR was tested for its specificity,sensitivity and repeatability.The result showed it could detect as low as 50 germs and with good specificity and repeatability and it could be finished within 7 hours.

关 键 词：猪链球菌2型 cps2J epf MRP 三重PCR 快速检测 

分 类 号：S852.611[农业科学—基础兽医学] TS207.3[农业科学—兽医学]