转染人乳头瘤病毒16型E6E7重组基因及感染模型细胞建立  

Transfection of Human Papillomavirus Type 16-E6E7 Gene and Establishment of an Infected Cell Line Model

作　　者：徐茜[1] 田训[1] 吴莺[1] 黄磊[1] 赵赟[1] 陈刚[1] 王世宣[1] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤生物医学中心妇产科,湖北武汉430030

出　　处：《病毒学报》2006年第2期144-146,共3页Chinese Journal of Virology

基　　金：国家自然科学基金(30271358);国家重点基础研究发展规划项目(973项目;2002CB513100)

摘　　要：To investigate the functions of HPV-16 E6E7 protein in the development of cervical cancer,a recombinant plasmid pEGFP-HPV16E6E7,containing green fluorescent protein and HPV-16 E6E7 proteins was constructed and was transfected into HPV-negative cell line C33A mediated by liposome.The efficiency of tranfection was determined by inverted fluorescent microscope and the expression of E6E7 was detected by Western blot.It showed that transfection of C33A cell and Caski cell by HPV-16 E6E7 gene established a pair of transfected cell types to study the carcinogenesis of E6 and E7 proteins.To investigate the functions of HPV-16 E6E7 protein in the development of cervical cancer, a recombinant plasmid pEGFP-HPV16E6E7, containing green fluorescent protein and HPV-16 E6E7 proteins was constructed and was transfected into HPV-negative cell line C33A mediated by liposome. The efficiency of tranfec- tion was determined by inverted fluorescent microscope and the expression of E6E7 was detected by Western blot. It showed that transfection of C33A cell and Caski cell by HPV-16 E6E7 gene established a pair of transfected cell types to study the carcinogenesis of E6 and E7 proteins.

关 键 词：人乳头病毒16型E6E7基因 基因转染 基因表达 

分 类 号：R373[医药卫生—病原生物学]