线虫基因组功能的高通量研究  

High-Throughput Genome of Caenorhabditis Elegans

作　　者：李怡璇[1] 汤华[1] 

机构地区：[1]天津医科大学天津市生命科学中心实验室,天津市300070

出　　处：《医学分子生物学杂志》2006年第2期122-125,共4页Journal of Medical Molecular Biology

摘　　要：线虫是研究细胞生物学的一个理想模型,RNA i现象也是最早在线虫中发现的。由于RNA i技术不仅具有高度特异性靶定基因的特点,而且简便、易于操作,从而成为研究基因组功能的高通量方法。很多研究小组已经使用大规模RNA i系统地研究线虫各个基因的功能缺失表型。线虫独特的RNA i方法和以此为基础的基因组大规模分析有利于对线虫的各种生物学过程产生新的认识。Caenorhabditis elegans serves as a good model organism for the study of cell biology, and the phenomenon of RNA-mediated interference （RNAi） was first discovered in the nematode Caenorhabditis elegans. RNA interference can target gene specifically, and it is simple, easy to perform. A high-throughput, genome-wide function analysis has been developed on the basis of RNAi technique. Several research teams used large-scale RNAi to systematically examine various C. elegans gene for knock-down phenotypes. The specific RNAi method and functional genomic analyses by high-throughput RNAi have provided new insights in a wide variety of biological processes in C.

关 键 词：秀丽广杆线虫 RNA干扰 高通量分析 

分 类 号：Q74[生物学—分子生物学]